Rapid method for the preparation of an AGE-BSA standard calibrator using thermal glycation

A. D. Bhatwadekar, V. S. Ghole

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Estimation of advanced glycation end products (AGEs) by determining fluorescence is based on the use of a standard calibrator prepared by incubating bovine serum albumin (BSA) and glucose at 37°C for 60 days. In the present study we attempted to reduce the duration of incubation to 4 days by increasing the temperature to 50°C. It is noteworthy that incubation at 50°C resulted in the rapid production of an AGE-BSA standard calibrator within 4 days. Amino-guanidine reduced the intensity of the glycation-induced fluorescence, while the addition of lysine intensified the reaction, as shown by the calibrator incubated at 37°C. The protein carbonyl content was shown to increase in the rapidly-formed standard calibrator. Thus we conclude that a simple increase in temperature and the addition of lysine (0.1M) can accelerate the process of glycation-induced fluorescence. This calibrator can be used effectively in fluorescence assays of AGEs.

Original languageEnglish (US)
Pages (from-to)11-15
Number of pages5
JournalJournal of Clinical Laboratory Analysis
Volume19
Issue number1
DOIs
StatePublished - Feb 9 2005
Externally publishedYes

Keywords

  • AGE-BSA
  • Advanced glycation end products
  • Diabetes
  • Fluorescence
  • Glycation
  • Lysine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

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