Rat hindleg perfusion: correlation of bone marrow leukocyte cellularity with release

G. Van Zant, Hal Broxmeyer, J. LoBue, A. S. Gordon

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The ability of isolated perfused hindlegs of vinblastine sulfate (VLB) treated rats to release leukocytes into leukocyte depleted blood was studied. Neutrophil release and bone marrow neutrophil population size were strongly correlated (r = 0.82) during the 17 day period after a subcutaneous injection of 0.7 or 1.0 mg VLB per kg body weight. During this period marrow neutrophils ranged from 0 on day 4 to 12.73 x 105/mg femoral marrow on day 10. Marrow lymphocyte population size and lymphocyte release were correlated (r = 0.70) during the first 6 days after VLB administration, when marrow lymphocyte numbers were depressed and starting to recover, but not during the subsequent 11 days. Unlike marrow neutrophils, marrow lymphocyte numbers did not rebound to above normal levels at any time during the 17 days studied, nor were they as severely depressed by VLB. They ranged from 1.56 x 105/mg femoral marrow on day 3 to a near normal level of 8.12 x 105/mg femoral marrow on day 17. Normal blood neutrophil numbers were maintained during the first 2 days after VLB but fell off sharply to less than 10% of normal on day 3. Apparently the marrow granulocyte reserve, although depleted to about 15% of normal on days 1 and 2, was nonetheless able to sustain normal circulating neutrophil numbers.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology
Pages1327-1332
Number of pages6
Volume225
Edition6
StatePublished - 1973
Externally publishedYes

Fingerprint

Leukocytes
Perfusion
Bone Marrow
Neutrophils
Thigh
Lymphocyte Count
Population Density
Lymphocytes
Vinblastine
Subcutaneous Injections
Granulocytes
Body Weight

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Van Zant, G., Broxmeyer, H., LoBue, J., & Gordon, A. S. (1973). Rat hindleg perfusion: correlation of bone marrow leukocyte cellularity with release. In American Journal of Physiology (6 ed., Vol. 225, pp. 1327-1332)

Rat hindleg perfusion : correlation of bone marrow leukocyte cellularity with release. / Van Zant, G.; Broxmeyer, Hal; LoBue, J.; Gordon, A. S.

American Journal of Physiology. Vol. 225 6. ed. 1973. p. 1327-1332.

Research output: Chapter in Book/Report/Conference proceedingChapter

Van Zant, G, Broxmeyer, H, LoBue, J & Gordon, AS 1973, Rat hindleg perfusion: correlation of bone marrow leukocyte cellularity with release. in American Journal of Physiology. 6 edn, vol. 225, pp. 1327-1332.
Van Zant G, Broxmeyer H, LoBue J, Gordon AS. Rat hindleg perfusion: correlation of bone marrow leukocyte cellularity with release. In American Journal of Physiology. 6 ed. Vol. 225. 1973. p. 1327-1332
Van Zant, G. ; Broxmeyer, Hal ; LoBue, J. ; Gordon, A. S. / Rat hindleg perfusion : correlation of bone marrow leukocyte cellularity with release. American Journal of Physiology. Vol. 225 6. ed. 1973. pp. 1327-1332
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