Rat insulin genes: Construction of plasmids containing the coding sequences

Axel Ullrich, John Shine, John Chirgwin, Raymond Pictet, Edmund Tischer, William J. Rutter, Howard M. Goodman

Research output: Contribution to journalArticle

808 Scopus citations

Abstract

Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3′nal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.

Original languageEnglish (US)
Pages (from-to)1313-1319
Number of pages7
JournalScience
Volume196
Issue number4296
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

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    Ullrich, A., Shine, J., Chirgwin, J., Pictet, R., Tischer, E., Rutter, W. J., & Goodman, H. M. (1977). Rat insulin genes: Construction of plasmids containing the coding sequences. Science, 196(4296), 1313-1319. https://doi.org/10.1126/science.325648