Rat model of arterial thrombosis induced by ferric chloride

K. D. Kurz, B. W. Main, George Sandusky

Research output: Contribution to journalArticle

351 Citations (Scopus)

Abstract

The purposes of these studies were to produce a small animal model of arterial thrombosis for study of novel antithrombotic agents, to validate a simple temperature index of occlusive thrombosis, and to describe the composition of the thrombus. Small thermocouple transducers were fabricated from readily available materials. A thermocouple was inserted under a carotid artery of the anesthetized rat and vessel temperature was recorded continuously. Arterial injury was induced by FeC13 solution applied topically to the artery above the thermocouple. To validate the relationship between thrombotic occlusion and vessel temperature, blood flow velocity, proximal to the injury, and temperature were recorded simultaneously. Temperature decreased rapidly when velocity averaged 24 ± 12 percent of control and velocity did not differ from zero within 20 sec. In normal vessels, average flow velocity did not decrease significantly from control until a fixed stenosis decreased diameter by 78 percent. Average time to occlusion (TTO), signaled by the abrupt temperature inflection, ranged from 56 ± 4 min to 14 ± 1 min after 10 and 65 percent FeC13 application respectively. Vessel segments were fixed at various times after FeC13 exposure and examined by scanning electron microscopy. Endothelial damage was observed and was associated with thrombus composed of activated platelets, fibrin strands and entrapped erythrocytes. The results demonstrate that FeC13 dose-dependently induced formation of an occlusive mixed thrombus that was indexed by monitoring the time between FeC13 application and a rapid temperature decrease in the carotid artery of the rat.

Original languageEnglish (US)
Pages (from-to)269-280
Number of pages12
JournalThrombosis Research
Volume60
Issue number4
DOIs
StatePublished - Nov 15 1990
Externally publishedYes

Fingerprint

Thrombosis
Temperature
Carotid Arteries
Fibrinolytic Agents
Blood Flow Velocity
Wounds and Injuries
ferric chloride
Fibrin
Transducers
Electron Scanning Microscopy
Pathologic Constriction
Blood Platelets
Animal Models
Arteries
Erythrocytes

Keywords

  • arterial thrombosis
  • ferric chloride
  • rat

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Rat model of arterial thrombosis induced by ferric chloride. / Kurz, K. D.; Main, B. W.; Sandusky, George.

In: Thrombosis Research, Vol. 60, No. 4, 15.11.1990, p. 269-280.

Research output: Contribution to journalArticle

Kurz, K. D. ; Main, B. W. ; Sandusky, George. / Rat model of arterial thrombosis induced by ferric chloride. In: Thrombosis Research. 1990 ; Vol. 60, No. 4. pp. 269-280.
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