Rat parotid gland cell differentiation in three-dimensional culture

Olga J. Baker, David J. Schulz, Jean M. Camden, Zhongji Liao, Troy S. Peterson, Cheikh Seye, Michael J. Petris, Gary A. Weisman

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The use of polarized salivary gland cell monolayers has contributed to our understanding of salivary gland physiology. However, these cell models are not representative of glandular epithelium in vivo, and, therefore, are not ideal for investigating salivary epithelial functions. The current study has developed a three-dimensional (3D) cell culture model for rat Par-C10 parotid gland cells that forms differentiated acinar-like spheres on Matrigel. These 3D Par-C10 acinar-like spheres display characteristics similar to differentiated acini in salivary glands, including cell polarization, tight junction (TJ) formation required to maintain transepithelial potential difference, basolateral expression of aquaporin-3 and Na+/K+/2Cl- cotransporter-1, and responsiveness to the muscarinic receptor agonist carbachol that is decreased by the anion channel blocker diphenylamine-2-carboxylic acid or chloride replacement with gluconate. Incubation of the spheres in the hypertonic medium increased the expression level of the water channel aquaporin-5. Further, the proinflammatory cytokines tumor necrosis factor-α and interferon-γ induced alterations in TJ integrity in the acinar-like spheres without affecting individual cell viability, suggesting that cytokines may affect salivary gland function by altering TJ integrity. Thus, 3D Par-C10 acinar-like spheres represent a novel in vitro model to study physiological and pathophysiological functions of differentiated acini.

Original languageEnglish (US)
Pages (from-to)1135-1144
Number of pages10
JournalTissue Engineering - Part C: Methods
Volume16
Issue number5
DOIs
StatePublished - Oct 1 2010
Externally publishedYes

Fingerprint

Parotid Gland
Salivary Glands
Rats
Tight Junctions
Cell Differentiation
Aquaporin 5
Aquaporin 3
Cytokines
Muscarinic Agonists
Aquaporins
Carbachol
Interferons
Muscarinic Receptors
Anions
Physiology
Chlorides
Cell Survival
Carboxylic acids
Cell culture
Epithelium

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)
  • Medicine(all)

Cite this

Baker, O. J., Schulz, D. J., Camden, J. M., Liao, Z., Peterson, T. S., Seye, C., ... Weisman, G. A. (2010). Rat parotid gland cell differentiation in three-dimensional culture. Tissue Engineering - Part C: Methods, 16(5), 1135-1144. https://doi.org/10.1089/ten.tec.2009.0438

Rat parotid gland cell differentiation in three-dimensional culture. / Baker, Olga J.; Schulz, David J.; Camden, Jean M.; Liao, Zhongji; Peterson, Troy S.; Seye, Cheikh; Petris, Michael J.; Weisman, Gary A.

In: Tissue Engineering - Part C: Methods, Vol. 16, No. 5, 01.10.2010, p. 1135-1144.

Research output: Contribution to journalArticle

Baker, OJ, Schulz, DJ, Camden, JM, Liao, Z, Peterson, TS, Seye, C, Petris, MJ & Weisman, GA 2010, 'Rat parotid gland cell differentiation in three-dimensional culture', Tissue Engineering - Part C: Methods, vol. 16, no. 5, pp. 1135-1144. https://doi.org/10.1089/ten.tec.2009.0438
Baker, Olga J. ; Schulz, David J. ; Camden, Jean M. ; Liao, Zhongji ; Peterson, Troy S. ; Seye, Cheikh ; Petris, Michael J. ; Weisman, Gary A. / Rat parotid gland cell differentiation in three-dimensional culture. In: Tissue Engineering - Part C: Methods. 2010 ; Vol. 16, No. 5. pp. 1135-1144.
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