Rate-determining steps in the biosynthesis of glycogen in COS cells

Alexander V. Skurat, Hwei Ling Peng, Hwan You Chang, John F. Cannon, Peter J. Roach

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Consistent with previous results, overexpression of rabbit skeletal muscle glycogen synthase in COS cells did not lead to overaccumulation of glycogen unless activating Ser → Ala mutations were present at key regulatory phosphorylation sites 2 (Ser7) and 3a (Ser644) in the enzyme. In addition, we found that expression of glycogenin, glycogen branching enzyme, or UDP-glucose pyrophosphorylase alone in COS cells had no effect on the glycogen level. However, coexpression of the hyperactive 2,3a glycogen synthase mutant with either glycogenin or UDP-glucose pyrophosphorylase led to higher glycogen accumulation than that obtained from the expression of glycogen synthase alone. Coexpression of glycogenin with the 2,3a mutant of glycogen synthase led to the appearance of glycogenin with a lower molecular weight suggestive of reduced glucosylation. Increased glycogen synthesis may lead to competition between glycogenin and glycogen synthase for their common substrate UDP-glucose. In summary, we conclude that (i) glycogen synthase is a primary rate-limiting enzyme of glycogen biosynthesis in COS cells, (ii) that phosphorylation of glycogen synthase is regulatory for glycogen accumulation, and (iii) once glycogen synthase is activated, the reaction mediated by UDP-glucose pyrophosphorylase can become rate-determining.

Original languageEnglish (US)
Pages (from-to)283-288
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume328
Issue number2
DOIs
StatePublished - Apr 15 1996

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Glycogen Synthase
COS Cells
Biosynthesis
Glycogen
UTP-Glucose-1-Phosphate Uridylyltransferase
Phosphorylation
1,4-alpha-Glucan Branching Enzyme
Uridine Diphosphate Glucose
Enzymes
Muscle
Skeletal Muscle
Molecular Weight
Molecular weight
glycogenin
Rabbits
Mutation
Substrates

Keywords

  • branching enzyme
  • glycogen
  • glycogen synthase
  • glycogenin
  • UDP-glucose pyrophosphorylase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Rate-determining steps in the biosynthesis of glycogen in COS cells. / Skurat, Alexander V.; Peng, Hwei Ling; Chang, Hwan You; Cannon, John F.; Roach, Peter J.

In: Archives of Biochemistry and Biophysics, Vol. 328, No. 2, 15.04.1996, p. 283-288.

Research output: Contribution to journalArticle

Skurat, Alexander V. ; Peng, Hwei Ling ; Chang, Hwan You ; Cannon, John F. ; Roach, Peter J. / Rate-determining steps in the biosynthesis of glycogen in COS cells. In: Archives of Biochemistry and Biophysics. 1996 ; Vol. 328, No. 2. pp. 283-288.
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