Rb-independent induction of apoptosis by bovine papillomavirus type 1 E7 in response to tumor necrosis factor α

Yun Liu, Yihui Hong, Elliot Androphy, Jason J. Chen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Bovine papillomavirus type 1 (BPV-1) is a small DNA virus that causes fibropapillomas of the host. BPV-1 has served as the prototype for studies of the molecular biology of the papillomaviruses. BPV-1 efficiently induces anchorage-independent growth and focus formation in murine C127 cells. The transforming properties of BPV-1 primarily reside in two genes, E5 and E6. Each of these genes is sufficient to transform cells. Although no independent transformation activity has been detected for E7, it was shown to be required for full transformation of C127 by BPV-1. We investigated the biologiced activities of BPV-1 E7 in several assays. Our results indicate that expression of BPV-1 E7 sensitizes cells to tumor necrosis factor α (TNF)-induced apoptosis. The TNF-induced apoptosis in E7-expressing cells was accompanied by increased release of arachidonic acid, indicating that phospholipase A2 was activated. Unlike the E7 proteins from the cancer-related human papillomaviruses, the BPV-1 E7 protein does not associate efficiently with the retinoblastoma protein (pRB) in vitro, nor does it significantly affect the pRB levels in cultured cells. Furthermore, BPV-1 E7 sensitizes Rb-null cells to TNF-induced apoptosis. These studies indicate that BPV-1 E7 can sensitize cells to apoptosis through mechanisms that are independent of pRB.

Original languageEnglish (US)
Pages (from-to)30894-30900
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number40
StatePublished - Oct 6 2000
Externally publishedYes

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Bovine papillomavirus 1
Tumor Necrosis Factor-alpha
Apoptosis
Genes
Retinoblastoma Protein
Molecular biology
Phospholipases A2
Viruses
Arachidonic Acid
Assays
Cells
DNA
Null Lymphocytes
DNA Viruses
Proteins
Molecular Biology
Cultured Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Rb-independent induction of apoptosis by bovine papillomavirus type 1 E7 in response to tumor necrosis factor α. / Liu, Yun; Hong, Yihui; Androphy, Elliot; Chen, Jason J.

In: Journal of Biological Chemistry, Vol. 275, No. 40, 06.10.2000, p. 30894-30900.

Research output: Contribution to journalArticle

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