Reactive oxygen species and the regulation of cell death by the Bcl-2 gene family

Stanley J. Korsmeyer, Xiao-Ming Yin, Zoltán N. Oltvai, Deborah J. Veis-Novack, Gerald P. Linette

Research output: Contribution to journalArticle

240 Citations (Scopus)

Abstract

The maintenance of homeostasis in normal tissues reflects a balance between cell proliferation and cell death. Bcl-2 inaugurated a new category of oncogenes, regulators of cell death. The Bcl-2 gene was identified at the chromosomal breakpoint of t(14;18) bearing B cell lymphomas. Bcl-2 proved unique by blocking programmed cell death rather than promoting proliferation. In adults, Bcl-2 is topographically restricted to progenitor cells and longlived cells but is much more widespread in the developing embryo. Transgenic mice that overexpress Bcl-2 demonstrate extended cell survival, and progress to high grade lymphomas. Bcl-2 has been localized to mitochondria, endoplasmic reticulum and nuclear membranes, also the sites of reactive oxygen species generation. Bcl-2 does not appear to influence the generation of oxygen free radicals but does prevent oxidative damage to cellular constituents including lipid membranes. Bcl-2 deficient mice complete embryonic development but undergo fulminant lymphoid apoptosis of thymus and spleen. Moreover, they demonstrate two unexpected pathologies resulting from cell death, polycystic kidney disease and hair hypopigmentation. The latter is a potential oxidant injury from the melanin biosynthetic pathway. A family of Bcl-2 related genes is emerging that includes Bax, a conserved homolog that heterodimerizes in vivo with Bcl-2 and promotes cell death. The ratio of family members, such as Bcl-2/Bax, determines the survival or death of cells following an apoptotic stimulus.

Original languageEnglish (US)
Pages (from-to)63-66
Number of pages4
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1271
Issue number1
DOIs
StatePublished - May 24 1995
Externally publishedYes

Fingerprint

bcl-2 Genes
Reactive Oxygen Species
Cell Death
Hypopigmentation
Polycystic Kidney Diseases
Biosynthetic Pathways
Melanins
Nuclear Envelope
B-Cell Lymphoma
Membrane Lipids
Oncogenes
Oxidants
Endoplasmic Reticulum
Hair
Non-Hodgkin's Lymphoma
Thymus Gland
Transgenic Mice
Free Radicals
Embryonic Development
Cell Survival

Keywords

  • Bcl-2
  • Cell death
  • Oncogene
  • Reactive oxygen species

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Biophysics

Cite this

Reactive oxygen species and the regulation of cell death by the Bcl-2 gene family. / Korsmeyer, Stanley J.; Yin, Xiao-Ming; Oltvai, Zoltán N.; Veis-Novack, Deborah J.; Linette, Gerald P.

In: Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol. 1271, No. 1, 24.05.1995, p. 63-66.

Research output: Contribution to journalArticle

Korsmeyer, Stanley J. ; Yin, Xiao-Ming ; Oltvai, Zoltán N. ; Veis-Novack, Deborah J. ; Linette, Gerald P. / Reactive oxygen species and the regulation of cell death by the Bcl-2 gene family. In: Biochimica et Biophysica Acta - Molecular Basis of Disease. 1995 ; Vol. 1271, No. 1. pp. 63-66.
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