Real-time studies of the progression of bacterial infections and immediate tissue responses in live animals

Lisa E. Månsson, Keira Melican, Jorrit Boekel, Ruben M. Sandoval, Isabelle Hautefort, George A. Tanner, Bruce Molitoris, Agneta Richter-dahlfors

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

By combining intravital multiphoton microscopy and bacterial genetics we have developed a technique enabling real-time imaging of bacterial proliferation and tissue responses in a live animal. Spatial and temporal control of the infection process was achieved by microinjecting GFP+-expressing uropathogenic Escherichiacoli (UPEC) into tubules of exteriorized kidneys in live rats. GFP+ was introduced in the clinical UPEC strain CFT073 as a single-copy chromosomal gene fusion. Within hours, bacterial colonization was accompanied by marked ischaemic effects, perivascular leakage, loss of tubular integrity and localized recruitment of immune cells. The pathophysiology was altered in response to an isogenic bacterial strain lacking the exotoxin haemolysin, revealing the subtle and temporal roles of bacterial virulence factors in vivo. Microdissection and RNA extraction of the injected nephron allowed molecular analysis of prokaryotic and eukaryotic gene expression. The techniques described here can be applied to study the integrated cell communication evoked by a variety of bacterial pathogens, assisting in the design of strategies to combat bacterial infections.

Original languageEnglish
Pages (from-to)413-424
Number of pages12
JournalCellular Microbiology
Volume9
Issue number2
DOIs
StatePublished - Feb 2007

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Time and motion study
Time and Motion Studies
Bacterial Infections
Animals
Microdissection
Tissue
Exotoxins
Hemolysin Proteins
Virulence Factors
Pathogens
Gene expression
Kidney Tubules
Rats
Microscopic examination
Fusion reactions
Genes
Gene Fusion
Nephrons
RNA
Infection Control

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Microbiology

Cite this

Månsson, L. E., Melican, K., Boekel, J., Sandoval, R. M., Hautefort, I., Tanner, G. A., ... Richter-dahlfors, A. (2007). Real-time studies of the progression of bacterial infections and immediate tissue responses in live animals. Cellular Microbiology, 9(2), 413-424. https://doi.org/10.1111/j.1462-5822.2006.00799.x

Real-time studies of the progression of bacterial infections and immediate tissue responses in live animals. / Månsson, Lisa E.; Melican, Keira; Boekel, Jorrit; Sandoval, Ruben M.; Hautefort, Isabelle; Tanner, George A.; Molitoris, Bruce; Richter-dahlfors, Agneta.

In: Cellular Microbiology, Vol. 9, No. 2, 02.2007, p. 413-424.

Research output: Contribution to journalArticle

Månsson, LE, Melican, K, Boekel, J, Sandoval, RM, Hautefort, I, Tanner, GA, Molitoris, B & Richter-dahlfors, A 2007, 'Real-time studies of the progression of bacterial infections and immediate tissue responses in live animals', Cellular Microbiology, vol. 9, no. 2, pp. 413-424. https://doi.org/10.1111/j.1462-5822.2006.00799.x
Månsson, Lisa E. ; Melican, Keira ; Boekel, Jorrit ; Sandoval, Ruben M. ; Hautefort, Isabelle ; Tanner, George A. ; Molitoris, Bruce ; Richter-dahlfors, Agneta. / Real-time studies of the progression of bacterial infections and immediate tissue responses in live animals. In: Cellular Microbiology. 2007 ; Vol. 9, No. 2. pp. 413-424.
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