Recapitulating Inner Ear Development with Pluripotent Stem Cells: Biology and Translation

Karl R. Koehler, Alexander K. Malone, Eri Hashino

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Since the formal inception of stem cell biology in the early 2000s, significant progress has been made to apply this new technology in the auditory and vestibular systems. Because mechanosensitive hair cells and sensory neurons, two major sensory cell types in the human inner ear, do not regenerate after their loss, cell replacement therapies have been considered as a viable option to cure profound hearing loss or balance disorders. In this chapter, we will overview how biological principles in inner ear development can be reconstituted in vitro to derive inner ear sensory cells and what criteria can be used to test the phenotypic and functional authenticity of these stem cell-derived cells. Additionally, an emerging new approach to create inner ear structures from aggregates of pluripotent stem cells in 3D culture will be discussed and compared with the conventional 2D culture system. Finally, ways that these technical advances could be translated into clinical therapy studies will be discussed.

Original languageEnglish (US)
Title of host publicationDevelopment of Auditory and Vestibular Systems
Subtitle of host publicationFourth Edition
PublisherElsevier Inc.
Pages213-247
Number of pages35
ISBN (Electronic)9780124081086
ISBN (Print)9780124080881
DOIs
StatePublished - Jun 2 2014

Keywords

  • Differentiation
  • Hair cells
  • Inner ear
  • Sensory neurons
  • Stem cells

ASJC Scopus subject areas

  • Medicine(all)
  • Neuroscience(all)

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    Koehler, K. R., Malone, A. K., & Hashino, E. (2014). Recapitulating Inner Ear Development with Pluripotent Stem Cells: Biology and Translation. In Development of Auditory and Vestibular Systems: Fourth Edition (pp. 213-247). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-408088-1.00008-7