Recent advances in genome editing and creation of genetically modified pigs

James R. Butler, Joseph M. Ladowski, Gregory R. Martens, Matthew Tector, A. Joseph Tector

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.

Original languageEnglish (US)
Pages (from-to)217-222
Number of pages6
JournalInternational Journal of Surgery
Volume23
DOIs
StatePublished - Nov 1 2015

Fingerprint

Genetic Engineering
Swine
Heterologous Transplantation
Endonucleases
Animal Models
Gene Editing

Keywords

  • Endonuclease
  • Homologous recombination
  • Off-target
  • TALEN CRISPR/Cas9
  • Zinc finger nuclease

ASJC Scopus subject areas

  • Surgery

Cite this

Butler, J. R., Ladowski, J. M., Martens, G. R., Tector, M., & Tector, A. J. (2015). Recent advances in genome editing and creation of genetically modified pigs. International Journal of Surgery, 23, 217-222. https://doi.org/10.1016/j.ijsu.2015.07.684

Recent advances in genome editing and creation of genetically modified pigs. / Butler, James R.; Ladowski, Joseph M.; Martens, Gregory R.; Tector, Matthew; Tector, A. Joseph.

In: International Journal of Surgery, Vol. 23, 01.11.2015, p. 217-222.

Research output: Contribution to journalArticle

Butler, James R. ; Ladowski, Joseph M. ; Martens, Gregory R. ; Tector, Matthew ; Tector, A. Joseph. / Recent advances in genome editing and creation of genetically modified pigs. In: International Journal of Surgery. 2015 ; Vol. 23. pp. 217-222.
@article{82842cc34c814095b0360d081f499d56,
title = "Recent advances in genome editing and creation of genetically modified pigs",
abstract = "The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.",
keywords = "Endonuclease, Homologous recombination, Off-target, TALEN CRISPR/Cas9, Zinc finger nuclease",
author = "Butler, {James R.} and Ladowski, {Joseph M.} and Martens, {Gregory R.} and Matthew Tector and Tector, {A. Joseph}",
year = "2015",
month = "11",
day = "1",
doi = "10.1016/j.ijsu.2015.07.684",
language = "English (US)",
volume = "23",
pages = "217--222",
journal = "International Journal of Surgery",
issn = "1743-9191",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Recent advances in genome editing and creation of genetically modified pigs

AU - Butler, James R.

AU - Ladowski, Joseph M.

AU - Martens, Gregory R.

AU - Tector, Matthew

AU - Tector, A. Joseph

PY - 2015/11/1

Y1 - 2015/11/1

N2 - The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.

AB - The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.

KW - Endonuclease

KW - Homologous recombination

KW - Off-target

KW - TALEN CRISPR/Cas9

KW - Zinc finger nuclease

UR - http://www.scopus.com/inward/record.url?scp=84951573938&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951573938&partnerID=8YFLogxK

U2 - 10.1016/j.ijsu.2015.07.684

DO - 10.1016/j.ijsu.2015.07.684

M3 - Article

VL - 23

SP - 217

EP - 222

JO - International Journal of Surgery

JF - International Journal of Surgery

SN - 1743-9191

ER -