Receptor-Mediated Endocytosis of Antigens Overcomes the Requirement for HLA-DM in Class II-Restricted Antigen Presentation

Ma Chenglie, Janice S. Blum

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

HLA-DM- APC are unable to present soluble Ags to T cells in the context of class II DR molecules. This defect in DM- APC can be overcome by receptor-mediated delivery of Ag into cells. Ag conjugated to ligands for cell surface receptors, such as transferrin or goat anti-human Ig, was processed and presented by DM- T2.DR4 cells. Intracellular processing of Ag conjugates was required, as receptor cross-linking alone did not restore presentation by DM- APC. Ag conjugates targeted by transferrin receptors to endosomes or via surface Ig to endosomal and lysosomal compartments, were each efficiently presented by DM- cells. These Ag conjugates were predominantly localized in light density endosomes in T2.DR4 cells. This study demonstrates that the facilitated up- take and sorting of exogenous Ag by cell surface receptors allow efficient class II-restricted presentation even in the absence of HLA-DM.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalJournal of Immunology
Volume158
Issue number1
StatePublished - Jan 1 1997

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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