Receptors for cholecystokinin and insulin in isolated pancreatic acini: Hormonal control of secretion and metabolism

J. A. Williams, H. Sankaran, Murray Korc, I. D. Goldfine

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Both isolated pancreatic cells and isolated pancreatic acini have been prepared from mouse and rat pancreas. Although isolated cells are useful for the study of early events in stimulus-secretion coupling such as regulation of Ca2+ fluxes, they show a relatively poor ability to secrete digestive enzymes in response to secretagogues. Most likely this deficiency in secretion is due to the loss of specialization at the luminal plasma membrane surface. In contrast to isolated cells, isolated pancreatic acini can also be used to study the secretion of digestive enzymes in response to hormones and neurotransmitters. Using isolated acini and a new preparation of radioiodinated cholecystokinin (CCK), we have been able to characterize pancreatic CCK receptors. Analysis of binding data reveals two orders of binding sites, a high affinity site (K(d)=64 pM) and a low affinity site (K(d) =21 nM). Stimulation of amylase release correlates with occupancy of the high affinity site. High affinity receptors for insulin are also present on isolated acini. After binding to these receptors, insulin stimulates protein and amylase synthesis. Insulin does not directly stimulate amylase secretion but rather potentiates the effects of hormonal secretagogues. These studies indicate, therefore, that isolated pancreatic acini can be employed to study in vitro the various events in hormonal and neurotransmitter regulation of pancreatic enzyme secretion and metabolism.

Original languageEnglish (US)
Pages (from-to)2497-2502
Number of pages6
JournalFederation Proceedings
Volume40
Issue number10
StatePublished - 1981
Externally publishedYes

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Cholecystokinin Receptors
Insulin Receptor
Amylases
Neurotransmitter Agents
Enzymes
Cholecystokinin
Pancreas
Binding Sites
Cell Membrane
Hormones
Insulin
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Receptors for cholecystokinin and insulin in isolated pancreatic acini : Hormonal control of secretion and metabolism. / Williams, J. A.; Sankaran, H.; Korc, Murray; Goldfine, I. D.

In: Federation Proceedings, Vol. 40, No. 10, 1981, p. 2497-2502.

Research output: Contribution to journalArticle

Williams, JA, Sankaran, H, Korc, M & Goldfine, ID 1981, 'Receptors for cholecystokinin and insulin in isolated pancreatic acini: Hormonal control of secretion and metabolism', Federation Proceedings, vol. 40, no. 10, pp. 2497-2502.
Williams, J. A. ; Sankaran, H. ; Korc, Murray ; Goldfine, I. D. / Receptors for cholecystokinin and insulin in isolated pancreatic acini : Hormonal control of secretion and metabolism. In: Federation Proceedings. 1981 ; Vol. 40, No. 10. pp. 2497-2502.
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