Recombinant human Interleukin-1 Alpha: A potent bio-immunomodifier in vivo in immunosuppressed mice induced by cyclophosphamide, retroviral infection and surgical stress

R. N. Shen, B. Wu, L. Lu, H. E. Kaiser, H. E. Broxmeyer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Recombinant human Interleukin-1 Alpha (rhu IL-1 α) was assessed for its efficacy in modifying the immunosuppression of mice compromised by Cyclophosphamide (CY), retrovirus infection or surgical stress. Sublethal dose (300 mg/kg) of CY caused neutropenia, decreased cellularity of bone marrow and inhibited Natural Killer (NK) cell activity and lymphokine-activated killer (LAK) cell activity in DBA/2 mice. A single dose of rhu IL-1 α (1000 units/per mouse) i.p. accelerated recovery of blood neutrophils and bone marrow cellularity and restored NK and LAK cell activity in CY-treated mice. Mice infected with Friend Virus Complex (FVC) had decreased percentages of L3T4+ cells and a reversed L3T4+/Lyt-2+ ratio; NK and LAK cell activity also decreased. These impaired cellular parameters were restored by rhu IL-1 α treatment (1000 units/per mouse/daily i.p. starting on day 5 for 5 days). NK and LAK cell activity was impaired by surgical stress. A single dose of rhu IL-1 α (1000 units/per mouse) i.p. 20 hours before transfemoral amputation restored NK and LAK cell activity to normal levels in these mice. These studies indicate that rhu IF-1 α possesses immunomodulatory effects in vivo for a broad range of stresses.

Original languageEnglish (US)
Pages (from-to)59-63
Number of pages5
JournalIn Vivo
Volume8
Issue number1
StatePublished - Jan 1 1994

Fingerprint

Interleukin-1alpha
Lymphokines
Cyclophosphamide
Lymphokine-Activated Killer Cells
Infection
Bone
Bone Marrow
Retroviridae Infections
Friend murine leukemia virus
Viruses
Inbred DBA Mouse
Blood
Neutropenia
Amputation
Natural Killer Cells
Immunosuppression
Recovery
Neutrophils

Keywords

  • cyclophosphamide
  • immunosuppressed mice
  • retrovirus
  • rhu IL-1 Alpha
  • surgical stress

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Recombinant human Interleukin-1 Alpha : A potent bio-immunomodifier in vivo in immunosuppressed mice induced by cyclophosphamide, retroviral infection and surgical stress. / Shen, R. N.; Wu, B.; Lu, L.; Kaiser, H. E.; Broxmeyer, H. E.

In: In Vivo, Vol. 8, No. 1, 01.01.1994, p. 59-63.

Research output: Contribution to journalArticle

@article{5d4087eb5c7c43fe852fedae5e6c4f0f,
title = "Recombinant human Interleukin-1 Alpha: A potent bio-immunomodifier in vivo in immunosuppressed mice induced by cyclophosphamide, retroviral infection and surgical stress",
abstract = "Recombinant human Interleukin-1 Alpha (rhu IL-1 α) was assessed for its efficacy in modifying the immunosuppression of mice compromised by Cyclophosphamide (CY), retrovirus infection or surgical stress. Sublethal dose (300 mg/kg) of CY caused neutropenia, decreased cellularity of bone marrow and inhibited Natural Killer (NK) cell activity and lymphokine-activated killer (LAK) cell activity in DBA/2 mice. A single dose of rhu IL-1 α (1000 units/per mouse) i.p. accelerated recovery of blood neutrophils and bone marrow cellularity and restored NK and LAK cell activity in CY-treated mice. Mice infected with Friend Virus Complex (FVC) had decreased percentages of L3T4+ cells and a reversed L3T4+/Lyt-2+ ratio; NK and LAK cell activity also decreased. These impaired cellular parameters were restored by rhu IL-1 α treatment (1000 units/per mouse/daily i.p. starting on day 5 for 5 days). NK and LAK cell activity was impaired by surgical stress. A single dose of rhu IL-1 α (1000 units/per mouse) i.p. 20 hours before transfemoral amputation restored NK and LAK cell activity to normal levels in these mice. These studies indicate that rhu IF-1 α possesses immunomodulatory effects in vivo for a broad range of stresses.",
keywords = "cyclophosphamide, immunosuppressed mice, retrovirus, rhu IL-1 Alpha, surgical stress",
author = "Shen, {R. N.} and B. Wu and L. Lu and Kaiser, {H. E.} and Broxmeyer, {H. E.}",
year = "1994",
month = "1",
day = "1",
language = "English (US)",
volume = "8",
pages = "59--63",
journal = "In Vivo",
issn = "0258-851X",
publisher = "International Institute of Anticancer Research",
number = "1",

}

TY - JOUR

T1 - Recombinant human Interleukin-1 Alpha

T2 - A potent bio-immunomodifier in vivo in immunosuppressed mice induced by cyclophosphamide, retroviral infection and surgical stress

AU - Shen, R. N.

AU - Wu, B.

AU - Lu, L.

AU - Kaiser, H. E.

AU - Broxmeyer, H. E.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Recombinant human Interleukin-1 Alpha (rhu IL-1 α) was assessed for its efficacy in modifying the immunosuppression of mice compromised by Cyclophosphamide (CY), retrovirus infection or surgical stress. Sublethal dose (300 mg/kg) of CY caused neutropenia, decreased cellularity of bone marrow and inhibited Natural Killer (NK) cell activity and lymphokine-activated killer (LAK) cell activity in DBA/2 mice. A single dose of rhu IL-1 α (1000 units/per mouse) i.p. accelerated recovery of blood neutrophils and bone marrow cellularity and restored NK and LAK cell activity in CY-treated mice. Mice infected with Friend Virus Complex (FVC) had decreased percentages of L3T4+ cells and a reversed L3T4+/Lyt-2+ ratio; NK and LAK cell activity also decreased. These impaired cellular parameters were restored by rhu IL-1 α treatment (1000 units/per mouse/daily i.p. starting on day 5 for 5 days). NK and LAK cell activity was impaired by surgical stress. A single dose of rhu IL-1 α (1000 units/per mouse) i.p. 20 hours before transfemoral amputation restored NK and LAK cell activity to normal levels in these mice. These studies indicate that rhu IF-1 α possesses immunomodulatory effects in vivo for a broad range of stresses.

AB - Recombinant human Interleukin-1 Alpha (rhu IL-1 α) was assessed for its efficacy in modifying the immunosuppression of mice compromised by Cyclophosphamide (CY), retrovirus infection or surgical stress. Sublethal dose (300 mg/kg) of CY caused neutropenia, decreased cellularity of bone marrow and inhibited Natural Killer (NK) cell activity and lymphokine-activated killer (LAK) cell activity in DBA/2 mice. A single dose of rhu IL-1 α (1000 units/per mouse) i.p. accelerated recovery of blood neutrophils and bone marrow cellularity and restored NK and LAK cell activity in CY-treated mice. Mice infected with Friend Virus Complex (FVC) had decreased percentages of L3T4+ cells and a reversed L3T4+/Lyt-2+ ratio; NK and LAK cell activity also decreased. These impaired cellular parameters were restored by rhu IL-1 α treatment (1000 units/per mouse/daily i.p. starting on day 5 for 5 days). NK and LAK cell activity was impaired by surgical stress. A single dose of rhu IL-1 α (1000 units/per mouse) i.p. 20 hours before transfemoral amputation restored NK and LAK cell activity to normal levels in these mice. These studies indicate that rhu IF-1 α possesses immunomodulatory effects in vivo for a broad range of stresses.

KW - cyclophosphamide

KW - immunosuppressed mice

KW - retrovirus

KW - rhu IL-1 Alpha

KW - surgical stress

UR - http://www.scopus.com/inward/record.url?scp=0028179751&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028179751&partnerID=8YFLogxK

M3 - Article

C2 - 8054512

AN - SCOPUS:0028179751

VL - 8

SP - 59

EP - 63

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 1

ER -