Recommendations for improved standardization of immunohistochemistry

Neal S. Goldstein, Stephen M. Hewitt, Clive R. Taylor, Hadi Yaziji, David G. Hicks, Ren L. Ridolfi, David J. Dabbs, Alvin W. Martin, Sunil Badve, Jan Hessling

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

Immunohistochemistry (IHC) continues to suffer from variable consistency, poor reproducibility, quality assurance disparities, and the lack of standardization resulting in poor concordance, validation, and verification. This document lists the recommendations made by the Ad-Hoc Committee on Immunohistochemistry Standardization to address these deficiencies. Contributing factors were established to be underfixation and irregular fixation, use of nonformalin fixatives and ancillary fixation procedures divested from a deep and full understanding of the IHC assay parameters, minimal or absent IHC assay optimization and validation procedures, and lack of a standard system of interpretation and reporting. Definitions and detailed guidelines pertaining to these areas are provided.

Original languageEnglish
Pages (from-to)124-133
Number of pages10
JournalApplied Immunohistochemistry and Molecular Morphology
Volume15
Issue number2
DOIs
StatePublished - Jun 2007

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Immunohistochemistry
Fixatives
Guidelines

Keywords

  • Assay
  • Fixation
  • Immunohistochemistry
  • Oncology
  • Pathology
  • Procedure
  • Standardization
  • Tissue

ASJC Scopus subject areas

  • Anatomy
  • Medical Laboratory Technology

Cite this

Goldstein, N. S., Hewitt, S. M., Taylor, C. R., Yaziji, H., Hicks, D. G., Ridolfi, R. L., ... Hessling, J. (2007). Recommendations for improved standardization of immunohistochemistry. Applied Immunohistochemistry and Molecular Morphology, 15(2), 124-133. https://doi.org/10.1097/PAI.0b013e31804c7283

Recommendations for improved standardization of immunohistochemistry. / Goldstein, Neal S.; Hewitt, Stephen M.; Taylor, Clive R.; Yaziji, Hadi; Hicks, David G.; Ridolfi, Ren L.; Dabbs, David J.; Martin, Alvin W.; Badve, Sunil; Hessling, Jan.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 15, No. 2, 06.2007, p. 124-133.

Research output: Contribution to journalArticle

Goldstein, NS, Hewitt, SM, Taylor, CR, Yaziji, H, Hicks, DG, Ridolfi, RL, Dabbs, DJ, Martin, AW, Badve, S & Hessling, J 2007, 'Recommendations for improved standardization of immunohistochemistry', Applied Immunohistochemistry and Molecular Morphology, vol. 15, no. 2, pp. 124-133. https://doi.org/10.1097/PAI.0b013e31804c7283
Goldstein, Neal S. ; Hewitt, Stephen M. ; Taylor, Clive R. ; Yaziji, Hadi ; Hicks, David G. ; Ridolfi, Ren L. ; Dabbs, David J. ; Martin, Alvin W. ; Badve, Sunil ; Hessling, Jan. / Recommendations for improved standardization of immunohistochemistry. In: Applied Immunohistochemistry and Molecular Morphology. 2007 ; Vol. 15, No. 2. pp. 124-133.
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