Recommendations for improved standardization of immunohistochemistry

Neal S. Goldstein, Stephen M. Hewitt, Clive R. Taylor, Hadi Yaziji, David G. Hicks, Ren L. Ridolfi, David J. Dabbs, Alvin W. Martin, Sunil Badve, Jan Hessling

Research output: Contribution to journalReview articlepeer-review

169 Scopus citations


Immunohistochemistry (IHC) continues to suffer from variable consistency, poor reproducibility, quality assurance disparities, and the lack of standardization resulting in poor concordance, validation, and verification. This document lists the recommendations made by the Ad-Hoc Committee on Immunohistochemistry Standardization to address these deficiencies. Contributing factors were established to be underfixation and irregular fixation, use of nonformalin fixatives and ancillary fixation procedures divested from a deep and full understanding of the IHC assay parameters, minimal or absent IHC assay optimization and validation procedures, and lack of a standard system of interpretation and reporting. Definitions and detailed guidelines pertaining to these areas are provided.

Original languageEnglish (US)
Pages (from-to)124-133
Number of pages10
JournalApplied Immunohistochemistry and Molecular Morphology
Issue number2
StatePublished - Jun 2007


  • Assay
  • Fixation
  • Immunohistochemistry
  • Oncology
  • Pathology
  • Procedure
  • Standardization
  • Tissue

ASJC Scopus subject areas

  • Anatomy
  • Medical Laboratory Technology

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