Reconstitution of hematopoiesis following transplantation into neonatal mice.

Scott A. Johnson, Mervin Yoder

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The primary sites of hematopoiesis change during murine ontogeny. The first blood cells emerge in two waves in the yolk sac; primitive erythroblasts, megakaryocytes, and macrophages emerge on embryonic d (E) 7.0, whereas definitive progenitor cells appear as clusters within the yolk sac vasculature on E8.25. Of interest, yolk sac cells isolated prior to d 10.5 fail to engraft in myeloablated adult recipient mice and do not reconstitute hematopoiesis. We describe a method of sublethally myeloablating newborn mice in which E9.0 yolk sac cells engraft and repopulate all lineages of the hematopoietic system for up to 12 mo in primary recipients and up to 6 mo in secondary recipients. The exact mechanisms that permit yolk sac engraftment in the conditioned newborn mice remain elusive, but this method has been used by a number of investigators to pursue transplantation studies using embryo- or fetal-derived donor cells.

Original languageEnglish
Pages (from-to)95-106
Number of pages12
JournalMethods in molecular medicine
Volume105
StatePublished - 2005

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Yolk Sac
Hematopoiesis
Transplantation
Hematopoietic System
Erythroblasts
Megakaryocytes
Blood Cells
Stem Cells
Embryonic Structures
Macrophages
Research Personnel

Cite this

Reconstitution of hematopoiesis following transplantation into neonatal mice. / Johnson, Scott A.; Yoder, Mervin.

In: Methods in molecular medicine, Vol. 105, 2005, p. 95-106.

Research output: Contribution to journalArticle

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