Reduced proliferation of endothelial colony-forming cells in unprovoked venous thromboembolic disease as a consequence of endothelial dysfunction

Rubicel Hernandez-Lopez, Antonieta Chavez-Gonzalez, Patricia Torres-Barrera, Dafne Moreno-Lorenzana, Norma Lopez-DiazGuerrero, David Santiago-German, Irma Isordia-Salas, David Smadja, Mervin Yoder, Abraham Majluf-Cruz, J. Antonio Alvarado-Moreno

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Abstract

Background: Venous thromboembolic disease (VTD) is a public health problem. We recently reported that endothelial colony-forming cells (ECFCs) derived from endothelial cells (EC) (ECFCECs) from patients with VTD have a dysfunctional state. For this study, we proposed that a dysfunctional status of these cells generates a reduction of its proliferative ability, which is also associated with senescence and reactive oxygen species (ROS). Methods and results: Human mononuclear cells (MNCs) were obtained from peripheral blood from 40 healthy human volunteers (controls) and 50 patients with VTD matched by age (20.50 years) and sex to obtain ECFCs. We assayed their proliferative ability with plasma of patients and controls and supernatants of cultures from ECFC-ECs, senescence-associated β-galactosidase (SA-β-gal), ROS, and expression of ephrin-B2/Eph-B4 receptor. Compared with cells from controls, cells from VTD patients showed an 8-fold increase of ECFCs that emerged 1 week earlier, reduced proliferation at long term (39%) and, in passages 4 and 10, a highly senescent rate (30±1.05% vs. 91.3±15.07%, respectively) with an increase of ROS and impaired expression of ephrin-B2/Eph-4 genes. Proliferation potential of cells from VTD patients was reduced in endothelial medium [1.4±0.22 doubling population (DP)], control plasma (1.18±0.31 DP), or plasma from VTD patients (1.65±0.27 DP). Conclusions: As compared with controls, ECFC-ECs from individuals with VTD have higher oxidative stress, proliferation stress, cellular senescence, and low proliferative potential. These findings suggest that patients with a history of VTD are ECFC-ECs dysfunctional that could be associated to permanent risk for new thrombotic events.

Original languageEnglish (US)
Article numbere0183827
JournalPLoS One
Volume12
Issue number9
DOIs
StatePublished - Sep 1 2017

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Ephrin-B2
cells
Reactive Oxygen Species
Cell Aging
Plasmas
reactive oxygen species
EphA1 Receptor
Galactosidases
Oxidative stress
Endothelial cells
Public health
Population Control
Medical problems
galactosidases
Population
Blood
Genes
Healthy Volunteers
Oxidative Stress
Endothelial Cells

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hernandez-Lopez, R., Chavez-Gonzalez, A., Torres-Barrera, P., Moreno-Lorenzana, D., Lopez-DiazGuerrero, N., Santiago-German, D., ... Alvarado-Moreno, J. A. (2017). Reduced proliferation of endothelial colony-forming cells in unprovoked venous thromboembolic disease as a consequence of endothelial dysfunction. PLoS One, 12(9), [e0183827]. https://doi.org/10.1371/journal.pone.0183827

Reduced proliferation of endothelial colony-forming cells in unprovoked venous thromboembolic disease as a consequence of endothelial dysfunction. / Hernandez-Lopez, Rubicel; Chavez-Gonzalez, Antonieta; Torres-Barrera, Patricia; Moreno-Lorenzana, Dafne; Lopez-DiazGuerrero, Norma; Santiago-German, David; Isordia-Salas, Irma; Smadja, David; Yoder, Mervin; Majluf-Cruz, Abraham; Alvarado-Moreno, J. Antonio.

In: PLoS One, Vol. 12, No. 9, e0183827, 01.09.2017.

Research output: Contribution to journalArticle

Hernandez-Lopez, R, Chavez-Gonzalez, A, Torres-Barrera, P, Moreno-Lorenzana, D, Lopez-DiazGuerrero, N, Santiago-German, D, Isordia-Salas, I, Smadja, D, Yoder, M, Majluf-Cruz, A & Alvarado-Moreno, JA 2017, 'Reduced proliferation of endothelial colony-forming cells in unprovoked venous thromboembolic disease as a consequence of endothelial dysfunction', PLoS One, vol. 12, no. 9, e0183827. https://doi.org/10.1371/journal.pone.0183827
Hernandez-Lopez R, Chavez-Gonzalez A, Torres-Barrera P, Moreno-Lorenzana D, Lopez-DiazGuerrero N, Santiago-German D et al. Reduced proliferation of endothelial colony-forming cells in unprovoked venous thromboembolic disease as a consequence of endothelial dysfunction. PLoS One. 2017 Sep 1;12(9). e0183827. https://doi.org/10.1371/journal.pone.0183827
Hernandez-Lopez, Rubicel ; Chavez-Gonzalez, Antonieta ; Torres-Barrera, Patricia ; Moreno-Lorenzana, Dafne ; Lopez-DiazGuerrero, Norma ; Santiago-German, David ; Isordia-Salas, Irma ; Smadja, David ; Yoder, Mervin ; Majluf-Cruz, Abraham ; Alvarado-Moreno, J. Antonio. / Reduced proliferation of endothelial colony-forming cells in unprovoked venous thromboembolic disease as a consequence of endothelial dysfunction. In: PLoS One. 2017 ; Vol. 12, No. 9.
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abstract = "Background: Venous thromboembolic disease (VTD) is a public health problem. We recently reported that endothelial colony-forming cells (ECFCs) derived from endothelial cells (EC) (ECFCECs) from patients with VTD have a dysfunctional state. For this study, we proposed that a dysfunctional status of these cells generates a reduction of its proliferative ability, which is also associated with senescence and reactive oxygen species (ROS). Methods and results: Human mononuclear cells (MNCs) were obtained from peripheral blood from 40 healthy human volunteers (controls) and 50 patients with VTD matched by age (20.50 years) and sex to obtain ECFCs. We assayed their proliferative ability with plasma of patients and controls and supernatants of cultures from ECFC-ECs, senescence-associated β-galactosidase (SA-β-gal), ROS, and expression of ephrin-B2/Eph-B4 receptor. Compared with cells from controls, cells from VTD patients showed an 8-fold increase of ECFCs that emerged 1 week earlier, reduced proliferation at long term (39{\%}) and, in passages 4 and 10, a highly senescent rate (30±1.05{\%} vs. 91.3±15.07{\%}, respectively) with an increase of ROS and impaired expression of ephrin-B2/Eph-4 genes. Proliferation potential of cells from VTD patients was reduced in endothelial medium [1.4±0.22 doubling population (DP)], control plasma (1.18±0.31 DP), or plasma from VTD patients (1.65±0.27 DP). Conclusions: As compared with controls, ECFC-ECs from individuals with VTD have higher oxidative stress, proliferation stress, cellular senescence, and low proliferative potential. These findings suggest that patients with a history of VTD are ECFC-ECs dysfunctional that could be associated to permanent risk for new thrombotic events.",
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AU - Hernandez-Lopez, Rubicel

AU - Chavez-Gonzalez, Antonieta

AU - Torres-Barrera, Patricia

AU - Moreno-Lorenzana, Dafne

AU - Lopez-DiazGuerrero, Norma

AU - Santiago-German, David

AU - Isordia-Salas, Irma

AU - Smadja, David

AU - Yoder, Mervin

AU - Majluf-Cruz, Abraham

AU - Alvarado-Moreno, J. Antonio

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