Reduced serotonergic immunoreactive fibers in the forebrain of alcohol- preferring rats

Feng Zhou, S. Bledsoe, L. Lumeng, T. K. Li

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Our previous study indicated that 5-hydroxytryptamine (5-HT) immunoreactive fiber densities were decreased in specific areas of the brain in alcohol-preferring rats (P) when compared with alcohol-nonpreferring rats (NP). The results of our current study show that there are quantitative and qualitative differences in 5-HT innervation in other selected regions of the forebrains of P rats. The 5-HT fiber density in the brains of young adult P and NP rats was measured by immunocytochemistry and quantitative image analysis. A routine error of two-dimensional quantitation of nerve fiber was addressed and an adjustment was made. The amount of 5-HT fibers was significantly lower in CA4 and fasciola cinereum of the dorsal hippocampus, caudate-putamen, and hypothalamus of the P as compared with NP rats (unpaired Student's t tests). In examining the fiber types, we found that, in the frontal cortical and hippocampal regions, where normally fine 5-HT fibers with small varicosities and thick 5-HT fibers with large varicosities coexist, fewer fine 5-HT fibers were seen in P rats as compared with NP rats. The fine fibers are known to be vulnerable to abusive drugs. These observations indicate that (a) there are quantitative differences in 5-HT innervation or that the 5-HT in some 5-HT fibers is reduced to a level undetectable by immunocytochemistry, and (b) the fine 5-HT fibers are specifically reduced to a greater degree in the selected brain regions of P rats when compared with that of NP rats. The involvement of the 5-HT system in the alcohol abuse is discussed.

Original languageEnglish
Pages (from-to)571-579
Number of pages9
JournalAlcoholism: Clinical and Experimental Research
Volume18
Issue number3
DOIs
StatePublished - 1994

Fingerprint

Prosencephalon
Rats
Serotonin
Alcohols
Fibers
Brain
Immunohistochemistry
Fasciola
Social Adjustment
Putamen
Nerve Fibers
Image analysis
Alcoholism
Hypothalamus
Young Adult
Hippocampus
Students

Keywords

  • Alcoholism
  • Animal Model
  • Image Analysis
  • Immunocytochemistry
  • Serotonin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Reduced serotonergic immunoreactive fibers in the forebrain of alcohol- preferring rats. / Zhou, Feng; Bledsoe, S.; Lumeng, L.; Li, T. K.

In: Alcoholism: Clinical and Experimental Research, Vol. 18, No. 3, 1994, p. 571-579.

Research output: Contribution to journalArticle

@article{b77a6471f7e2487ab430a2a1ca6f43e7,
title = "Reduced serotonergic immunoreactive fibers in the forebrain of alcohol- preferring rats",
abstract = "Our previous study indicated that 5-hydroxytryptamine (5-HT) immunoreactive fiber densities were decreased in specific areas of the brain in alcohol-preferring rats (P) when compared with alcohol-nonpreferring rats (NP). The results of our current study show that there are quantitative and qualitative differences in 5-HT innervation in other selected regions of the forebrains of P rats. The 5-HT fiber density in the brains of young adult P and NP rats was measured by immunocytochemistry and quantitative image analysis. A routine error of two-dimensional quantitation of nerve fiber was addressed and an adjustment was made. The amount of 5-HT fibers was significantly lower in CA4 and fasciola cinereum of the dorsal hippocampus, caudate-putamen, and hypothalamus of the P as compared with NP rats (unpaired Student's t tests). In examining the fiber types, we found that, in the frontal cortical and hippocampal regions, where normally fine 5-HT fibers with small varicosities and thick 5-HT fibers with large varicosities coexist, fewer fine 5-HT fibers were seen in P rats as compared with NP rats. The fine fibers are known to be vulnerable to abusive drugs. These observations indicate that (a) there are quantitative differences in 5-HT innervation or that the 5-HT in some 5-HT fibers is reduced to a level undetectable by immunocytochemistry, and (b) the fine 5-HT fibers are specifically reduced to a greater degree in the selected brain regions of P rats when compared with that of NP rats. The involvement of the 5-HT system in the alcohol abuse is discussed.",
keywords = "Alcoholism, Animal Model, Image Analysis, Immunocytochemistry, Serotonin",
author = "Feng Zhou and S. Bledsoe and L. Lumeng and Li, {T. K.}",
year = "1994",
doi = "10.1111/j.1530-0277.1994.tb00912.x",
language = "English",
volume = "18",
pages = "571--579",
journal = "Alcoholism: Clinical and Experimental Research",
issn = "0145-6008",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Reduced serotonergic immunoreactive fibers in the forebrain of alcohol- preferring rats

AU - Zhou, Feng

AU - Bledsoe, S.

AU - Lumeng, L.

AU - Li, T. K.

PY - 1994

Y1 - 1994

N2 - Our previous study indicated that 5-hydroxytryptamine (5-HT) immunoreactive fiber densities were decreased in specific areas of the brain in alcohol-preferring rats (P) when compared with alcohol-nonpreferring rats (NP). The results of our current study show that there are quantitative and qualitative differences in 5-HT innervation in other selected regions of the forebrains of P rats. The 5-HT fiber density in the brains of young adult P and NP rats was measured by immunocytochemistry and quantitative image analysis. A routine error of two-dimensional quantitation of nerve fiber was addressed and an adjustment was made. The amount of 5-HT fibers was significantly lower in CA4 and fasciola cinereum of the dorsal hippocampus, caudate-putamen, and hypothalamus of the P as compared with NP rats (unpaired Student's t tests). In examining the fiber types, we found that, in the frontal cortical and hippocampal regions, where normally fine 5-HT fibers with small varicosities and thick 5-HT fibers with large varicosities coexist, fewer fine 5-HT fibers were seen in P rats as compared with NP rats. The fine fibers are known to be vulnerable to abusive drugs. These observations indicate that (a) there are quantitative differences in 5-HT innervation or that the 5-HT in some 5-HT fibers is reduced to a level undetectable by immunocytochemistry, and (b) the fine 5-HT fibers are specifically reduced to a greater degree in the selected brain regions of P rats when compared with that of NP rats. The involvement of the 5-HT system in the alcohol abuse is discussed.

AB - Our previous study indicated that 5-hydroxytryptamine (5-HT) immunoreactive fiber densities were decreased in specific areas of the brain in alcohol-preferring rats (P) when compared with alcohol-nonpreferring rats (NP). The results of our current study show that there are quantitative and qualitative differences in 5-HT innervation in other selected regions of the forebrains of P rats. The 5-HT fiber density in the brains of young adult P and NP rats was measured by immunocytochemistry and quantitative image analysis. A routine error of two-dimensional quantitation of nerve fiber was addressed and an adjustment was made. The amount of 5-HT fibers was significantly lower in CA4 and fasciola cinereum of the dorsal hippocampus, caudate-putamen, and hypothalamus of the P as compared with NP rats (unpaired Student's t tests). In examining the fiber types, we found that, in the frontal cortical and hippocampal regions, where normally fine 5-HT fibers with small varicosities and thick 5-HT fibers with large varicosities coexist, fewer fine 5-HT fibers were seen in P rats as compared with NP rats. The fine fibers are known to be vulnerable to abusive drugs. These observations indicate that (a) there are quantitative differences in 5-HT innervation or that the 5-HT in some 5-HT fibers is reduced to a level undetectable by immunocytochemistry, and (b) the fine 5-HT fibers are specifically reduced to a greater degree in the selected brain regions of P rats when compared with that of NP rats. The involvement of the 5-HT system in the alcohol abuse is discussed.

KW - Alcoholism

KW - Animal Model

KW - Image Analysis

KW - Immunocytochemistry

KW - Serotonin

UR - http://www.scopus.com/inward/record.url?scp=0028365749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028365749&partnerID=8YFLogxK

U2 - 10.1111/j.1530-0277.1994.tb00912.x

DO - 10.1111/j.1530-0277.1994.tb00912.x

M3 - Article

VL - 18

SP - 571

EP - 579

JO - Alcoholism: Clinical and Experimental Research

JF - Alcoholism: Clinical and Experimental Research

SN - 0145-6008

IS - 3

ER -