Reduced T-lymphocyte cell reactivity as a function of human aging

J. P. Antel, J. J F Oger, Edward Dropcho, D. P. Richman, H. H. Kuo, B. G. Arnason

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The mechanisms underlying reduced T-cell mitogenic reactivity which accompany human aging were explored in vitro using concanavalin A (Con A) to induce cell proliferation. Peak mitogenic reactivity of both unfractionated mononuclear cells (MNCs) and purified E+ cells from elderly donors was reduced compared to the corresponding cell populations from young individuals. Flow cytometric studies demonstrated that the number of cells entered into the cell cycle at the time of maximum mitogenic stimulation was reduced in the elderly. Peak response to Con A of E+ cells from young donors cocultured with monocytes from old individuals matched that obtained when these cells were cocultured with monocytes from young donors. The response to Con A of cocultures of monocytes from young donors and E+ cells of old donors merely matched the reactivity of old donors' MNCs. These data indicate that changes in intrinsic T-cell properties alone account for the reduced proliferative capacity in the elderly.

Original languageEnglish (US)
Pages (from-to)184-192
Number of pages9
JournalCellular Immunology
Volume54
Issue number1
DOIs
StatePublished - 1980
Externally publishedYes

Fingerprint

T-Lymphocytes
Tissue Donors
Concanavalin A
Monocytes
Coculture Techniques
Cell Cycle
Cell Count
Cell Proliferation
Population

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Antel, J. P., Oger, J. J. F., Dropcho, E., Richman, D. P., Kuo, H. H., & Arnason, B. G. (1980). Reduced T-lymphocyte cell reactivity as a function of human aging. Cellular Immunology, 54(1), 184-192. https://doi.org/10.1016/0008-8749(80)90200-2

Reduced T-lymphocyte cell reactivity as a function of human aging. / Antel, J. P.; Oger, J. J F; Dropcho, Edward; Richman, D. P.; Kuo, H. H.; Arnason, B. G.

In: Cellular Immunology, Vol. 54, No. 1, 1980, p. 184-192.

Research output: Contribution to journalArticle

Antel, JP, Oger, JJF, Dropcho, E, Richman, DP, Kuo, HH & Arnason, BG 1980, 'Reduced T-lymphocyte cell reactivity as a function of human aging', Cellular Immunology, vol. 54, no. 1, pp. 184-192. https://doi.org/10.1016/0008-8749(80)90200-2
Antel, J. P. ; Oger, J. J F ; Dropcho, Edward ; Richman, D. P. ; Kuo, H. H. ; Arnason, B. G. / Reduced T-lymphocyte cell reactivity as a function of human aging. In: Cellular Immunology. 1980 ; Vol. 54, No. 1. pp. 184-192.
@article{5c8d43d7644e43e09583efbd821050e4,
title = "Reduced T-lymphocyte cell reactivity as a function of human aging",
abstract = "The mechanisms underlying reduced T-cell mitogenic reactivity which accompany human aging were explored in vitro using concanavalin A (Con A) to induce cell proliferation. Peak mitogenic reactivity of both unfractionated mononuclear cells (MNCs) and purified E+ cells from elderly donors was reduced compared to the corresponding cell populations from young individuals. Flow cytometric studies demonstrated that the number of cells entered into the cell cycle at the time of maximum mitogenic stimulation was reduced in the elderly. Peak response to Con A of E+ cells from young donors cocultured with monocytes from old individuals matched that obtained when these cells were cocultured with monocytes from young donors. The response to Con A of cocultures of monocytes from young donors and E+ cells of old donors merely matched the reactivity of old donors' MNCs. These data indicate that changes in intrinsic T-cell properties alone account for the reduced proliferative capacity in the elderly.",
author = "Antel, {J. P.} and Oger, {J. J F} and Edward Dropcho and Richman, {D. P.} and Kuo, {H. H.} and Arnason, {B. G.}",
year = "1980",
doi = "10.1016/0008-8749(80)90200-2",
language = "English (US)",
volume = "54",
pages = "184--192",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Reduced T-lymphocyte cell reactivity as a function of human aging

AU - Antel, J. P.

AU - Oger, J. J F

AU - Dropcho, Edward

AU - Richman, D. P.

AU - Kuo, H. H.

AU - Arnason, B. G.

PY - 1980

Y1 - 1980

N2 - The mechanisms underlying reduced T-cell mitogenic reactivity which accompany human aging were explored in vitro using concanavalin A (Con A) to induce cell proliferation. Peak mitogenic reactivity of both unfractionated mononuclear cells (MNCs) and purified E+ cells from elderly donors was reduced compared to the corresponding cell populations from young individuals. Flow cytometric studies demonstrated that the number of cells entered into the cell cycle at the time of maximum mitogenic stimulation was reduced in the elderly. Peak response to Con A of E+ cells from young donors cocultured with monocytes from old individuals matched that obtained when these cells were cocultured with monocytes from young donors. The response to Con A of cocultures of monocytes from young donors and E+ cells of old donors merely matched the reactivity of old donors' MNCs. These data indicate that changes in intrinsic T-cell properties alone account for the reduced proliferative capacity in the elderly.

AB - The mechanisms underlying reduced T-cell mitogenic reactivity which accompany human aging were explored in vitro using concanavalin A (Con A) to induce cell proliferation. Peak mitogenic reactivity of both unfractionated mononuclear cells (MNCs) and purified E+ cells from elderly donors was reduced compared to the corresponding cell populations from young individuals. Flow cytometric studies demonstrated that the number of cells entered into the cell cycle at the time of maximum mitogenic stimulation was reduced in the elderly. Peak response to Con A of E+ cells from young donors cocultured with monocytes from old individuals matched that obtained when these cells were cocultured with monocytes from young donors. The response to Con A of cocultures of monocytes from young donors and E+ cells of old donors merely matched the reactivity of old donors' MNCs. These data indicate that changes in intrinsic T-cell properties alone account for the reduced proliferative capacity in the elderly.

UR - http://www.scopus.com/inward/record.url?scp=0018890187&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018890187&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(80)90200-2

DO - 10.1016/0008-8749(80)90200-2

M3 - Article

C2 - 6967772

AN - SCOPUS:0018890187

VL - 54

SP - 184

EP - 192

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -