Region-specific growth properties and trophic requirements of brain- and spinal cord-derived rat embryonic neural precursor cells

S. L. Fu, Z. W. Ma, L. Yin, C. Iannotti, P. H. Lu, Xiao-Ming Xu

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To determine whether neural precursor cells have region-specific growth properties, we compared the proliferation, mitogenicity, and differentiation of these cells isolated from the embryonic day 16 rat forebrain and spinal cord. Neural precursor cells isolated from both regions were cultured in growth medium supplemented with epidermal growth factor, basic fibroblast growth factor, or epidermal growth factor+basic fibroblast growth factor. Under all three conditions, both neural precursor cell populations proliferated for multiple passages. While spinal cord-derived neural precursor cells proliferated moderately faster in epidermal growth factor-enriched growth medium, brain-derived cells proliferated much faster in basic fibroblast growth factor-enriched growth medium. When exposed to both epidermal growth factor and basic fibroblast growth factor, the two neural precursor cell populations expanded and proliferated more rapidly than when exposed to a single factor, with brain-derived neural precursor cells expanding significantly faster than spinal cord-derived ones (P

Original languageEnglish (US)
Pages (from-to)851-862
Number of pages12
JournalNeuroscience
Volume135
Issue number3
DOIs
StatePublished - 2005
Externally publishedYes

Fingerprint

Spinal Cord
Fibroblast Growth Factor 2
Epidermal Growth Factor
Brain
Growth
Prosencephalon
Population
Cell Differentiation

Keywords

  • Brain
  • Differentiation
  • Embryonic
  • Neural progenitor cells
  • Neural stem cells
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Region-specific growth properties and trophic requirements of brain- and spinal cord-derived rat embryonic neural precursor cells. / Fu, S. L.; Ma, Z. W.; Yin, L.; Iannotti, C.; Lu, P. H.; Xu, Xiao-Ming.

In: Neuroscience, Vol. 135, No. 3, 2005, p. 851-862.

Research output: Contribution to journalArticle

Fu, S. L. ; Ma, Z. W. ; Yin, L. ; Iannotti, C. ; Lu, P. H. ; Xu, Xiao-Ming. / Region-specific growth properties and trophic requirements of brain- and spinal cord-derived rat embryonic neural precursor cells. In: Neuroscience. 2005 ; Vol. 135, No. 3. pp. 851-862.
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