Regional central nervous system densities of delta-opioid receptors in alcohol-preferring P, alcohol-nonpreferring NP, and unselected Wistar rats

Wendy N. Strother, Eyassu J. Chernet, Larry Lumeng, Ting Kai Li, William J. McBride

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The densities of delta-opioid receptors in the central nervous system of alcohol-naive, adult, male, alcohol-preferring P, alcohol-nonpreferring NP, and Wistar rats were examined with the use of quantitative autoradiography. Slides with coronal 20-μm sections through the regions of interest were incubated in 5 nM [3H]-[D-Pen2,D-Pen5]enkephalin (DPDPE) to label delta1-opioid receptor sites. Nonspecific binding was determined in the presence of 10 μM naloxone. Significant differences between the P and the NP rat lines were found in numerous cortical regions, the basolateral amygdala, and the posterior hippocampus, with 10%-20% lower [3H]-DPDPE binding found in the P line. In most regions examined, binding levels in the Wistar rats were intermediate between those of the P and the NP rats. Significantly lower [3H]-DPDPE binding levels in the P rat may indicate fewer delta1-opioid receptors or decreased binding affinity. The lower binding in certain limbic regions, such as the basolateral amygdala and posterior hippocampus, as well as cortical differences in the P rat may be associated with the divergent alcohol drinking behaviors found between the P and the NP lines.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalAlcohol
Volume25
Issue number1
DOIs
StatePublished - 2001

Fingerprint

delta Opioid Receptor
D-Penicillamine (2,5)-Enkephalin
Neurology
Wistar Rats
Rats
Central Nervous System
alcohol
Alcohols
Opioid Receptors
Hippocampus
Drinking Behavior
Naloxone
Autoradiography
Alcohol Drinking
Labels
Basolateral Nuclear Complex

Keywords

  • [H]-DPDPE
  • Alcohol drinking
  • Autoradiography
  • Delta-opioid receptor
  • Selected rat lines

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neuroscience(all)
  • Toxicology
  • Health(social science)

Cite this

Regional central nervous system densities of delta-opioid receptors in alcohol-preferring P, alcohol-nonpreferring NP, and unselected Wistar rats. / Strother, Wendy N.; Chernet, Eyassu J.; Lumeng, Larry; Li, Ting Kai; McBride, William J.

In: Alcohol, Vol. 25, No. 1, 2001, p. 31-38.

Research output: Contribution to journalArticle

Strother, Wendy N. ; Chernet, Eyassu J. ; Lumeng, Larry ; Li, Ting Kai ; McBride, William J. / Regional central nervous system densities of delta-opioid receptors in alcohol-preferring P, alcohol-nonpreferring NP, and unselected Wistar rats. In: Alcohol. 2001 ; Vol. 25, No. 1. pp. 31-38.
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