Regional CNS densities of serotonin1A and dopamine D2 receptors in periadolescent alcohol-preferring P and alcohol-nonpreferring NP rat pups

Wendy N. Strother, Larry Lumeng, Ting Kai Li, William J. McBride

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The objective of the present study was to use quantitative autoradiography to determine binding densities of serotonin1A (5-HT1A) and dopamine (DA) D2 receptors in alcohol-naive periadolescent P and NP rat pups. P (n=8) and NP (n=7) rat pups, 25 days of age, from different litters were used. Coronal brain sections were incubated with 2 nM [3H]8-OH-DPAT or 20 nM [3H]sulpiride for 5-HT1A or D2 binding, respectively. Approximately 15-40% higher densities of [3H]8-OH-DPAT binding were observed in the anterior cortical regions of the periadolescent P rat compared with NP rat pups. Similar differences were also observed in posterior cortical regions with P rats having 25-40% higher [3H]8-OH-DPAT binding than NP rats. [3H]8-OH-DPAT binding was approximately 10-20% higher in posterior hippocampal regions of the P rat pups compared with the NP line. [3H]sulpiride binding was significantly different only in the ventral tegmental area (VTA), where binding was approximately 20% lower in the periadolescent P rats compared with the NP rat pups. Overall, these results are very similar to findings observed in adult alcohol-naive P and NP rats, and suggest that the innate differences in the neural systems implicated in high alcohol drinking behaviors may already be established in the periadolescent animal.

Original languageEnglish (US)
Pages (from-to)335-342
Number of pages8
JournalPharmacology Biochemistry and Behavior
Volume74
Issue number2
DOIs
StatePublished - Jan 1 2003

Keywords

  • 5-HT receptors
  • Alcohol-nonpreferring rats
  • Alcohol-preferring rats
  • Autoradiography
  • D receptors
  • Periadolescence

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

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