Regional densities of benzodiazepine sites in the CNS of alcohol-naive P and NP rats

R. J. Thielen, W. J. McBride, E. Chernet, L. Lumeng, T. K. Li

Research output: Contribution to journalArticle

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Abstract

The regional densities of benzodiazepine (BDZ) recognition sites coupled to GABA, receptors were studied in ethanol-naive alcohol-preferring (P) and -nonpreferring (NP) lines of rats by using quantitative autoradiography to measure the amount of 2 nM [3H]flunitrazepam (FNZ) binding in the absence and presence of 100 μM GABA. Lower values (p < 0.025) for [3H]FNZ binding (in the absence of GABA) were observed in the prefrontal cortex, layer 4 of the parietal cortex, and the nucleus accumbens shell of the P relative to the NP line. GABA significantly (p < 0.025) stimulated [3H]FNZ binding in all 50 central nervous system regions examined in both the P and the NP rats. The largest percent increases (190-220%) were observed in the prefrontal, cingulate, frontal, and parietal cortices; shell and core nucleus accumbens; caudate putamen; dorsal lateral, intermediate lateral, ventral lateral, and medial septal nuclei; and lateral hypothalamus. In several layers of the frontal and parietal cortices, a 25-30% greater net or percent increase (p < 0.025) in GABA-enhanced [3H]FNZ binding was observed in the P rats compared with the NP rats. In contrast, lower net or percent increases (p < 0.025) in GABA-enhanced [3H]FNZ binding were found in the entorhinal cortex, the mediodorsal thalamus, and the dorsal CA3 area and middle dentate gyrus of the posterior hippocampus of the P line relative to the NP line. The present findings suggest that there are innate regional differences between P and NP rats in the densities and/or affinities of BDZ recognition sites and in the coupling between the GABA, and BDZ binding sites.

Original languageEnglish
Pages (from-to)875-882
Number of pages8
JournalPharmacology Biochemistry and Behavior
Volume57
Issue number4
DOIs
StatePublished - Aug 1997

Fingerprint

Flunitrazepam
Benzodiazepines
gamma-Aminobutyric Acid
Rats
Alcohols
Parietal Lobe
Septal Nuclei
Nucleus Accumbens
Frontal Lobe
Prefrontal Cortex
Lateral Hypothalamic Area
Parahippocampal Gyrus
Entorhinal Cortex
GABA Receptors
Putamen
Gyrus Cinguli
Dentate Gyrus
Neurology
Autoradiography
Thalamus

Keywords

  • Alcohol-preferring rats
  • Autoradiography
  • Benzodiazepine recognition sites
  • Flunitrazepam binding
  • GABA(A) receptors

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this

Regional densities of benzodiazepine sites in the CNS of alcohol-naive P and NP rats. / Thielen, R. J.; McBride, W. J.; Chernet, E.; Lumeng, L.; Li, T. K.

In: Pharmacology Biochemistry and Behavior, Vol. 57, No. 4, 08.1997, p. 875-882.

Research output: Contribution to journalArticle

Thielen, R. J. ; McBride, W. J. ; Chernet, E. ; Lumeng, L. ; Li, T. K. / Regional densities of benzodiazepine sites in the CNS of alcohol-naive P and NP rats. In: Pharmacology Biochemistry and Behavior. 1997 ; Vol. 57, No. 4. pp. 875-882.
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