Regional distribution of lysosomal enzymes in the canine retinal pigment epithelium

L. Cabral, W. Unger, M. Boulton, R. Lightfoot, N. McKechnie, I. Grierson, J. Marshall

Research output: Contribution to journalArticle

23 Scopus citations


We have investigated the activities of four lysosomal enzymes in RPE cells isolated from three regions of the canine fundus: the tapetal area, the central pigmented area and the peripheral area. The results obtained with fresly isolated cells showed that the activities of acid phosphatase, B-glucuronidase and N-acetyl-B-glucosaminidase were significantly higher in RPE cells derived from the peripheral region when compared to those from the two central regions. In contrast, the activity of cathepsin D was significantly higher in the tapetal region than in the periphery. The regional distribution of both acid phosphatase and B-glucuronidase observed in fresh RPE cells was progresively lost when these cells were grown in culture. Estimations of photoreceptor density per RPE cell from each of the regions indicated that the number of photoreceptors per RPE cell did not vary significantly with retinal location and suggested that variations in enzyme content were not relateed to differences in photoreceptor cell distribution.

Original languageEnglish (US)
Pages (from-to)670-676
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Issue number4
StatePublished - 1990


  • canine
  • lysosomal enzymes
  • regional distribution
  • retinal pigment epithelium

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Cabral, L., Unger, W., Boulton, M., Lightfoot, R., McKechnie, N., Grierson, I., & Marshall, J. (1990). Regional distribution of lysosomal enzymes in the canine retinal pigment epithelium. Investigative Ophthalmology and Visual Science, 31(4), 670-676.