Regional protein alterations in rat kidneys induced by lead exposure

Frank Witzmann, Carla D. Fultz, Raymond A. Grant, Linda S. Wright, Steven E. Kornguth, Frank L. Siegel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lead is a potent neuro- and nephrotoxin in humans and a renal carcinogen in rats. Previous studies have detected lead-induced increases in the activities of specific detoxification enzymes in distinct kidney cell types preceding irreversible renal damage. While preferential susceptibility of the highly vascularized cortex to the effects of lead is clear, lead effects on the medullary region have remained unexplored. The present study was undertaken to investigate the extent to which regional renal protein expression differs and to determine which, if any, regionally distinct protein markers indicative of lead's renotoxic mechanism might be detected in kidney cortical and medullary cytosols. We examined protein expression in these two functionally and anatomically distinct regions, and identified several proteins that are differentially expressed in those regions and were significantly altered by lead. Kidney cytosols from rats injected with lead acetate (114 mg/kg, three consecutive daily injections) were separated by two-dimensional electrophoresis. Lead exposure significantly (P

Original languageEnglish (US)
Title of host publicationFrom Genome to Proteome: Advances in the Practice & Application of Proteomics
PublisherWiley Blackwell
Pages363-371
Number of pages9
ISBN (Print)9783527613489, 9783527301546
DOIs
StatePublished - Dec 26 2007

Fingerprint

Rats
Kidney
Proteins
Cytosol
Detoxification
Neurotoxins
Electrophoresis
Carcinogens
Lead
Injections
Enzymes

Keywords

  • Cortex
  • Cytoplasm
  • Kidney
  • Lead
  • Medulla
  • Proteomics
  • Rat
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Witzmann, F., Fultz, C. D., Grant, R. A., Wright, L. S., Kornguth, S. E., & Siegel, F. L. (2007). Regional protein alterations in rat kidneys induced by lead exposure. In From Genome to Proteome: Advances in the Practice & Application of Proteomics (pp. 363-371). Wiley Blackwell. https://doi.org/10.1002/9783527613489.ch47

Regional protein alterations in rat kidneys induced by lead exposure. / Witzmann, Frank; Fultz, Carla D.; Grant, Raymond A.; Wright, Linda S.; Kornguth, Steven E.; Siegel, Frank L.

From Genome to Proteome: Advances in the Practice & Application of Proteomics. Wiley Blackwell, 2007. p. 363-371.

Research output: Chapter in Book/Report/Conference proceedingChapter

Witzmann, F, Fultz, CD, Grant, RA, Wright, LS, Kornguth, SE & Siegel, FL 2007, Regional protein alterations in rat kidneys induced by lead exposure. in From Genome to Proteome: Advances in the Practice & Application of Proteomics. Wiley Blackwell, pp. 363-371. https://doi.org/10.1002/9783527613489.ch47
Witzmann F, Fultz CD, Grant RA, Wright LS, Kornguth SE, Siegel FL. Regional protein alterations in rat kidneys induced by lead exposure. In From Genome to Proteome: Advances in the Practice & Application of Proteomics. Wiley Blackwell. 2007. p. 363-371 https://doi.org/10.1002/9783527613489.ch47
Witzmann, Frank ; Fultz, Carla D. ; Grant, Raymond A. ; Wright, Linda S. ; Kornguth, Steven E. ; Siegel, Frank L. / Regional protein alterations in rat kidneys induced by lead exposure. From Genome to Proteome: Advances in the Practice & Application of Proteomics. Wiley Blackwell, 2007. pp. 363-371
@inbook{0f207c315acb43959521c412872e3ecb,
title = "Regional protein alterations in rat kidneys induced by lead exposure",
abstract = "Lead is a potent neuro- and nephrotoxin in humans and a renal carcinogen in rats. Previous studies have detected lead-induced increases in the activities of specific detoxification enzymes in distinct kidney cell types preceding irreversible renal damage. While preferential susceptibility of the highly vascularized cortex to the effects of lead is clear, lead effects on the medullary region have remained unexplored. The present study was undertaken to investigate the extent to which regional renal protein expression differs and to determine which, if any, regionally distinct protein markers indicative of lead's renotoxic mechanism might be detected in kidney cortical and medullary cytosols. We examined protein expression in these two functionally and anatomically distinct regions, and identified several proteins that are differentially expressed in those regions and were significantly altered by lead. Kidney cytosols from rats injected with lead acetate (114 mg/kg, three consecutive daily injections) were separated by two-dimensional electrophoresis. Lead exposure significantly (P",
keywords = "Cortex, Cytoplasm, Kidney, Lead, Medulla, Proteomics, Rat, Two-dimensional electrophoresis",
author = "Frank Witzmann and Fultz, {Carla D.} and Grant, {Raymond A.} and Wright, {Linda S.} and Kornguth, {Steven E.} and Siegel, {Frank L.}",
year = "2007",
month = "12",
day = "26",
doi = "10.1002/9783527613489.ch47",
language = "English (US)",
isbn = "9783527613489",
pages = "363--371",
booktitle = "From Genome to Proteome: Advances in the Practice & Application of Proteomics",
publisher = "Wiley Blackwell",

}

TY - CHAP

T1 - Regional protein alterations in rat kidneys induced by lead exposure

AU - Witzmann, Frank

AU - Fultz, Carla D.

AU - Grant, Raymond A.

AU - Wright, Linda S.

AU - Kornguth, Steven E.

AU - Siegel, Frank L.

PY - 2007/12/26

Y1 - 2007/12/26

N2 - Lead is a potent neuro- and nephrotoxin in humans and a renal carcinogen in rats. Previous studies have detected lead-induced increases in the activities of specific detoxification enzymes in distinct kidney cell types preceding irreversible renal damage. While preferential susceptibility of the highly vascularized cortex to the effects of lead is clear, lead effects on the medullary region have remained unexplored. The present study was undertaken to investigate the extent to which regional renal protein expression differs and to determine which, if any, regionally distinct protein markers indicative of lead's renotoxic mechanism might be detected in kidney cortical and medullary cytosols. We examined protein expression in these two functionally and anatomically distinct regions, and identified several proteins that are differentially expressed in those regions and were significantly altered by lead. Kidney cytosols from rats injected with lead acetate (114 mg/kg, three consecutive daily injections) were separated by two-dimensional electrophoresis. Lead exposure significantly (P

AB - Lead is a potent neuro- and nephrotoxin in humans and a renal carcinogen in rats. Previous studies have detected lead-induced increases in the activities of specific detoxification enzymes in distinct kidney cell types preceding irreversible renal damage. While preferential susceptibility of the highly vascularized cortex to the effects of lead is clear, lead effects on the medullary region have remained unexplored. The present study was undertaken to investigate the extent to which regional renal protein expression differs and to determine which, if any, regionally distinct protein markers indicative of lead's renotoxic mechanism might be detected in kidney cortical and medullary cytosols. We examined protein expression in these two functionally and anatomically distinct regions, and identified several proteins that are differentially expressed in those regions and were significantly altered by lead. Kidney cytosols from rats injected with lead acetate (114 mg/kg, three consecutive daily injections) were separated by two-dimensional electrophoresis. Lead exposure significantly (P

KW - Cortex

KW - Cytoplasm

KW - Kidney

KW - Lead

KW - Medulla

KW - Proteomics

KW - Rat

KW - Two-dimensional electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=84961322376&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961322376&partnerID=8YFLogxK

U2 - 10.1002/9783527613489.ch47

DO - 10.1002/9783527613489.ch47

M3 - Chapter

SN - 9783527613489

SN - 9783527301546

SP - 363

EP - 371

BT - From Genome to Proteome: Advances in the Practice & Application of Proteomics

PB - Wiley Blackwell

ER -