Regional protein alterations in rat kidneys induced by lead exposure

Frank Witzmann, Carla D. Fultz, Raymond A. Grant, Linda S. Wright, Steven E. Kornguth, Frank L. Siegel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lead is a potent neuro- and nephrotoxin in humans and a renal carcinogen in rats. Previous studies have detected lead-induced increases in the activities of specific detoxification enzymes in distinct kidney cell types preceding irreversible renal damage. While preferential susceptibility of the highly vascularized cortex to the effects of lead is clear, lead effects on the medullary region have remained unexplored. The present study was undertaken to investigate the extent to which regional renal protein expression differs and to determine which, if any, regionally distinct protein markers indicative of lead's renotoxic mechanism might be detected in kidney cortical and medullary cytosols. We examined protein expression in these two functionally and anatomically distinct regions, and identified several proteins that are differentially expressed in those regions and were significantly altered by lead. Kidney cytosols from rats injected with lead acetate (114 mg/kg, three consecutive daily injections) were separated by two-dimensional electrophoresis. Lead exposure significantly (P

Original languageEnglish (US)
Title of host publicationFrom Genome to Proteome: Advances in the Practice & Application of Proteomics
PublisherWiley Blackwell
Pages363-371
Number of pages9
ISBN (Print)9783527613489, 9783527301546
DOIs
StatePublished - Dec 26 2007

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Keywords

  • Cortex
  • Cytoplasm
  • Kidney
  • Lead
  • Medulla
  • Proteomics
  • Rat
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Witzmann, F., Fultz, C. D., Grant, R. A., Wright, L. S., Kornguth, S. E., & Siegel, F. L. (2007). Regional protein alterations in rat kidneys induced by lead exposure. In From Genome to Proteome: Advances in the Practice & Application of Proteomics (pp. 363-371). Wiley Blackwell. https://doi.org/10.1002/9783527613489.ch47