Regional reproducibility of pulsed arterial spin labeling perfusion imaging at 3T

Yang Wang, Andrew J. Saykin, Josef Pfeuffer, Chen Lin, Kristine M. Mosier, Li Shen, Sungeun Kim, Gary D. Hutchins

Research output: Contribution to journalArticle

62 Scopus citations


Arterial spin labeling (ASL) is a promising non-invasive magnetic resonance imaging (MRI) technique for measuring regional cerebral blood flow (rCBF) or perfusion in vivo. To evaluate the feasibility of ASL as a biomarker for clinical trials, it is important to examine test-retest reproducibility. We investigated both inter- and intra-session reproducibility of perfusion MRI using a pulsed ASL (PASL) sequence PICORE Q2TIPS with an echo-planar imaging (EPI) readout. Structural MRI regions of interest (ROIs) were extracted individually by automated parcellation and segmentation methods using FreeSurfer. These cortical and subcortical ROIs were used to assess regional perfusion stability. Our results indicated regional variability in grey matter rCBF. Although rCBF measurements were characterized by intersubject variation, our results also indicated relatively less within-subject variability estimated as within-subject standard deviation (SDW) (intersession SDW: 2.0 to 8.8; intrasession SDW: 2.8 to 9.6) and acceptable reliabilities as measured using intraclass correlation coefficient (ICC) (intersession ICC: 0.68 to 0.94; intrasession ICC: 0.66 to 0.95) for regional MRI perfusion measurements using the PICORE Q2TIPS technique. Overall, our findings suggest that PASL is a technique with good within and between session reproducibility. Further reproducibility studies in target populations relevant for specific clinical trials of neurovascular related agents will be important and the present results provide a framework for such assessments.

Original languageEnglish (US)
Pages (from-to)1188-1195
Number of pages8
Issue number2
StatePublished - Jan 15 2011


  • Arterial spin labelling
  • Cerebral blood flow
  • Magnetic resonance imaging
  • Reproducibility

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Neurology

Fingerprint Dive into the research topics of 'Regional reproducibility of pulsed arterial spin labeling perfusion imaging at 3T'. Together they form a unique fingerprint.

  • Cite this