Regions of intrinsic disorder help identify a novel nuclear localization signal in Toxoplasma gondii histone acetyltransferase TgGCN5-B

Stacy E. Dixon, Micah M. Bhatti, Vladimir N. Uversky, A. Keith Dunker, William J. Sullivan

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We have previously shown that protozoan parasites, such as Toxoplasma gondii, contain a high prevalence of intrinsically disordered regions in their predicted proteins. Here, we determine that both TgGCN5-family histone acetyltransferases (HATs) contain unusually high levels of intrinsic disorder. A previously identified basic-rich nuclear localization signal (NLS) in the N-terminus of TgGCN5-A is located within such a region of predicted disorder, but this NLS is not conserved in TgGCN5-B. We therefore analyzed the intrinsically disordered regions of TgGCN5-B for basic-rich sequences that could be indicative of a functional NLS, and this led to the identification of a novel NLS for TgGCN5-B, RPAENKKRGR. The functionality of the GCN5-B NLS was validated experimentally and has predictive value. These studies demonstrate that basic-rich sequences within regions predicted to be intrinsically disordered constitute criteria for a candidate NLS.

Original languageEnglish (US)
Pages (from-to)192-195
Number of pages4
JournalMolecular and Biochemical Parasitology
Volume175
Issue number2
DOIs
StatePublished - Feb 1 2011

Keywords

  • Apicomplexa
  • Cellular trafficking
  • Chromatin
  • Epigenetics
  • GCN5
  • Parasite

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

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