regSNPs-splicing: a tool for prioritizing synonymous single-nucleotide substitution

Xinjun Zhang, Meng Li, Hai Lin, Xi Rao, Weixing Feng, Yuedong Yang, Matthew Mort, David N. Cooper, Yue Wang, Yadong Wang, Clark Wells, Yaoqi Zhou, Yunlong Liu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

While synonymous single-nucleotide variants (sSNVs) have largely been unstudied, since they do not alter protein sequence, mounting evidence suggests that they may affect RNA conformation, splicing, and the stability of nascent-mRNAs to promote various diseases. Accurately prioritizing deleterious sSNVs from a pool of neutral ones can significantly improve our ability of selecting functional genetic variants identified from various genome-sequencing projects, and, therefore, advance our understanding of disease etiology. In this study, we develop a computational algorithm to prioritize sSNVs based on their impact on mRNA splicing and protein function. In addition to genomic features that potentially affect splicing regulation, our proposed algorithm also includes dozens structural features that characterize the functions of alternatively spliced exons on protein function. Our systematical evaluation on thousands of sSNVs suggests that several structural features, including intrinsic disorder protein scores, solvent accessible surface areas, protein secondary structures, and known and predicted protein family domains, show significant differences between disease-causing and neutral sSNVs. Our result suggests that the protein structure features offer an added dimension of information while distinguishing disease-causing and neutral synonymous variants. The inclusion of structural features increases the predictive accuracy for functional sSNV prioritization.

Original languageEnglish (US)
Pages (from-to)1279-1289
Number of pages11
JournalHuman genetics
Volume136
Issue number9
DOIs
StatePublished - Sep 1 2017

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Zhang, X., Li, M., Lin, H., Rao, X., Feng, W., Yang, Y., Mort, M., Cooper, D. N., Wang, Y., Wang, Y., Wells, C., Zhou, Y., & Liu, Y. (2017). regSNPs-splicing: a tool for prioritizing synonymous single-nucleotide substitution. Human genetics, 136(9), 1279-1289. https://doi.org/10.1007/s00439-017-1783-x