Regulation and role of nuclear receptors during larval molting and metamorphosis of lepidoptera

Lynn M. Riddiford, Kiyoshi Hiruma, Que Lan, Baohua Zhou

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

SYNOPSIS. The regulation of the ecdysteroid-induced cascade of transcription factors by 20-hydroxyecdysone (20E) and juvenile hormone (JH) in the epidermis of the lepidopteran Manduca sexta is' summarized. Both the levels and isoforms of the ecdysone receptor (EcR) and Ultraspiracle (USP) change during the molt. The 20E induction of MHR3, an orphan receptor that appears relatively late in the cascade, is dependent on the predominant intermolt isoforms, EcR-Bl and USP-1, and on the orphan receptor E75A which appears early in the cascade. The USP-2 isoform induced by 20E pairs with EcR-Bl but this heterodimer cannot up-regulate MHR3. Thus, the specific transcription factors induced by 20E are dependent on the particular isoforms present in the heterodimeric EcR/USP complex. Juvenile hormone modulates responses of the transcription factor cascade to 20E. For E75A, the presence of JH increased both its sensitivity to 20E and the accumulation of its mRNA, indicating that high E75A may be important for maintenance of the larval state. By contrast, JH prevented the 20E induction of the Broad Complex group of transcription factors which appear as the larval epidermis becomes pupal ly committed and are necessary for metamorphosis in Drosophila. During the decline of ecdjsteroid, the orphan receptors, GRF and FTZ-Fl, appear and may regulate transcription of dopa decarboxylase, a key enzyme in sclerotization of the new cuticle. Thus, the ecdysteroid-induced regulatory cascade is set in motion and regulated by the changing concentration and types of ecdysteroids and receptor isoforms. The presence of JH further modulates this cascade by acting selectively on the responses of specific factors and thus prevents the 20E-induced gene switching necessary for metamorphosis.

Original languageEnglish (US)
Pages (from-to)736-746
Number of pages11
JournalAmerican Zoologist
Volume39
Issue number4
StatePublished - 1999
Externally publishedYes

Fingerprint

juvenile hormones
metamorphosis
hormone
molting
orphan
Lepidoptera
ecdysteroids
transcription factors
receptors
epidermis (animal)
sclerotization
ecdysterone
Manduca sexta
cuticle
molt
Drosophila
transcription (genetics)
regulation
enzyme
ecdysone receptor

ASJC Scopus subject areas

  • Environmental Science(all)
  • Earth and Planetary Sciences(all)

Cite this

Regulation and role of nuclear receptors during larval molting and metamorphosis of lepidoptera. / Riddiford, Lynn M.; Hiruma, Kiyoshi; Lan, Que; Zhou, Baohua.

In: American Zoologist, Vol. 39, No. 4, 1999, p. 736-746.

Research output: Contribution to journalArticle

Riddiford, Lynn M. ; Hiruma, Kiyoshi ; Lan, Que ; Zhou, Baohua. / Regulation and role of nuclear receptors during larval molting and metamorphosis of lepidoptera. In: American Zoologist. 1999 ; Vol. 39, No. 4. pp. 736-746.
@article{906130a9436640ec9b22060e50c73853,
title = "Regulation and role of nuclear receptors during larval molting and metamorphosis of lepidoptera",
abstract = "SYNOPSIS. The regulation of the ecdysteroid-induced cascade of transcription factors by 20-hydroxyecdysone (20E) and juvenile hormone (JH) in the epidermis of the lepidopteran Manduca sexta is' summarized. Both the levels and isoforms of the ecdysone receptor (EcR) and Ultraspiracle (USP) change during the molt. The 20E induction of MHR3, an orphan receptor that appears relatively late in the cascade, is dependent on the predominant intermolt isoforms, EcR-Bl and USP-1, and on the orphan receptor E75A which appears early in the cascade. The USP-2 isoform induced by 20E pairs with EcR-Bl but this heterodimer cannot up-regulate MHR3. Thus, the specific transcription factors induced by 20E are dependent on the particular isoforms present in the heterodimeric EcR/USP complex. Juvenile hormone modulates responses of the transcription factor cascade to 20E. For E75A, the presence of JH increased both its sensitivity to 20E and the accumulation of its mRNA, indicating that high E75A may be important for maintenance of the larval state. By contrast, JH prevented the 20E induction of the Broad Complex group of transcription factors which appear as the larval epidermis becomes pupal ly committed and are necessary for metamorphosis in Drosophila. During the decline of ecdjsteroid, the orphan receptors, GRF and FTZ-Fl, appear and may regulate transcription of dopa decarboxylase, a key enzyme in sclerotization of the new cuticle. Thus, the ecdysteroid-induced regulatory cascade is set in motion and regulated by the changing concentration and types of ecdysteroids and receptor isoforms. The presence of JH further modulates this cascade by acting selectively on the responses of specific factors and thus prevents the 20E-induced gene switching necessary for metamorphosis.",
author = "Riddiford, {Lynn M.} and Kiyoshi Hiruma and Que Lan and Baohua Zhou",
year = "1999",
language = "English (US)",
volume = "39",
pages = "736--746",
journal = "Integrative and Comparative Biology",
issn = "1540-7063",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Regulation and role of nuclear receptors during larval molting and metamorphosis of lepidoptera

AU - Riddiford, Lynn M.

AU - Hiruma, Kiyoshi

AU - Lan, Que

AU - Zhou, Baohua

PY - 1999

Y1 - 1999

N2 - SYNOPSIS. The regulation of the ecdysteroid-induced cascade of transcription factors by 20-hydroxyecdysone (20E) and juvenile hormone (JH) in the epidermis of the lepidopteran Manduca sexta is' summarized. Both the levels and isoforms of the ecdysone receptor (EcR) and Ultraspiracle (USP) change during the molt. The 20E induction of MHR3, an orphan receptor that appears relatively late in the cascade, is dependent on the predominant intermolt isoforms, EcR-Bl and USP-1, and on the orphan receptor E75A which appears early in the cascade. The USP-2 isoform induced by 20E pairs with EcR-Bl but this heterodimer cannot up-regulate MHR3. Thus, the specific transcription factors induced by 20E are dependent on the particular isoforms present in the heterodimeric EcR/USP complex. Juvenile hormone modulates responses of the transcription factor cascade to 20E. For E75A, the presence of JH increased both its sensitivity to 20E and the accumulation of its mRNA, indicating that high E75A may be important for maintenance of the larval state. By contrast, JH prevented the 20E induction of the Broad Complex group of transcription factors which appear as the larval epidermis becomes pupal ly committed and are necessary for metamorphosis in Drosophila. During the decline of ecdjsteroid, the orphan receptors, GRF and FTZ-Fl, appear and may regulate transcription of dopa decarboxylase, a key enzyme in sclerotization of the new cuticle. Thus, the ecdysteroid-induced regulatory cascade is set in motion and regulated by the changing concentration and types of ecdysteroids and receptor isoforms. The presence of JH further modulates this cascade by acting selectively on the responses of specific factors and thus prevents the 20E-induced gene switching necessary for metamorphosis.

AB - SYNOPSIS. The regulation of the ecdysteroid-induced cascade of transcription factors by 20-hydroxyecdysone (20E) and juvenile hormone (JH) in the epidermis of the lepidopteran Manduca sexta is' summarized. Both the levels and isoforms of the ecdysone receptor (EcR) and Ultraspiracle (USP) change during the molt. The 20E induction of MHR3, an orphan receptor that appears relatively late in the cascade, is dependent on the predominant intermolt isoforms, EcR-Bl and USP-1, and on the orphan receptor E75A which appears early in the cascade. The USP-2 isoform induced by 20E pairs with EcR-Bl but this heterodimer cannot up-regulate MHR3. Thus, the specific transcription factors induced by 20E are dependent on the particular isoforms present in the heterodimeric EcR/USP complex. Juvenile hormone modulates responses of the transcription factor cascade to 20E. For E75A, the presence of JH increased both its sensitivity to 20E and the accumulation of its mRNA, indicating that high E75A may be important for maintenance of the larval state. By contrast, JH prevented the 20E induction of the Broad Complex group of transcription factors which appear as the larval epidermis becomes pupal ly committed and are necessary for metamorphosis in Drosophila. During the decline of ecdjsteroid, the orphan receptors, GRF and FTZ-Fl, appear and may regulate transcription of dopa decarboxylase, a key enzyme in sclerotization of the new cuticle. Thus, the ecdysteroid-induced regulatory cascade is set in motion and regulated by the changing concentration and types of ecdysteroids and receptor isoforms. The presence of JH further modulates this cascade by acting selectively on the responses of specific factors and thus prevents the 20E-induced gene switching necessary for metamorphosis.

UR - http://www.scopus.com/inward/record.url?scp=22844455491&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22844455491&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:22844455491

VL - 39

SP - 736

EP - 746

JO - Integrative and Comparative Biology

JF - Integrative and Comparative Biology

SN - 1540-7063

IS - 4

ER -