Regulation by physical training of enzyme activity and gene expression of branched-chain 2-oxo acid dehydrogenase complex in rat skeletal muscle

Hisao Fujii, Kumpei Tokuyama, Masashige Suzuki, Kirill M. Popov, Yu Zhao, Robert Harris, Naoya Nakai, Taro Murakami, Yoshiharu Shimomura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We examined the effects of short-term (5 weeks) and long-term (12 weeks) physical training on actual and total activities, protein content and mRNA abundance of branched-chain 2-oxo acid dehydrogenase complex in rat skeletal muscle. The actual and total activities were significantly increased ∼ 60% and ∼ 40%, respectively, by long term training. No effects of short-term training on activities were observed. The increase in the total activity corresponded to increased protein content of the E1α and E2 components of the complex. On the other hand, mRNA abundance for E1α and E2 were not affected by the training, but that for E1β was slightly, but significantly increased by both short-term and long-term trainings. These divergent alterations of the message levels for the subunits of the complex suggest that posttranslational regulatory mechanisms determine the amount of the complex in skeletal muscle. Since the complex is located in the mitochondrial matrix space, mitochondrial biogenesis in response to the training was examined by determining the content of mitochondrial DNA in the muscle. The mitochondrial DNA was proportionally increased with the total activity as well as the protein content of the complex, suggesting that expression of branched-chain 2-oxo acid dehydrogenase complex in skeletal muscle in response to physical training is associated with mitochondrial biogenesis.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalBiochimica et Biophysica Acta - General Subjects
Volume1243
Issue number2
DOIs
StatePublished - Feb 23 1995

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3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Enzyme activity
Gene expression
Muscle
Rats
Teaching
Skeletal Muscle
Organelle Biogenesis
Mitochondrial DNA
Gene Expression
Enzymes
Messenger RNA
Proteins
Muscles

Keywords

  • (Rat skeletal muscle)
  • Branched-chain 2-oxo acid dehydrogenase complex
  • Mitochondrial DNA
  • mRNA
  • Physical training

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Regulation by physical training of enzyme activity and gene expression of branched-chain 2-oxo acid dehydrogenase complex in rat skeletal muscle. / Fujii, Hisao; Tokuyama, Kumpei; Suzuki, Masashige; Popov, Kirill M.; Zhao, Yu; Harris, Robert; Nakai, Naoya; Murakami, Taro; Shimomura, Yoshiharu.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1243, No. 2, 23.02.1995, p. 277-281.

Research output: Contribution to journalArticle

Fujii, Hisao ; Tokuyama, Kumpei ; Suzuki, Masashige ; Popov, Kirill M. ; Zhao, Yu ; Harris, Robert ; Nakai, Naoya ; Murakami, Taro ; Shimomura, Yoshiharu. / Regulation by physical training of enzyme activity and gene expression of branched-chain 2-oxo acid dehydrogenase complex in rat skeletal muscle. In: Biochimica et Biophysica Acta - General Subjects. 1995 ; Vol. 1243, No. 2. pp. 277-281.
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