Abstract
Objective: Adiponectin mRNA expression in isolated subcutaneous and omental adipocytes was examined across a wide range of adiposity to determine whether adiponectin synthesis is impaired in these adipose tissue depots in obese humans. Tumor necrosis factor (TNF)α and dexamethasone were tested for inhibitory effects on adiponectin release from human adipocytes in vitro. Research Methods and Procedures: Adipocytes were isolated by collagenase digestion of abdominal adipose tissue obtained from subjects undergoing surgical procedures or outpatient needle biopsy. Adiponectin and leptin mRNA were quantitated by real-time reverse transcriptase-polymerase chain reaction. Adiponectin and leptin secretion from isolated adipocytes treated with dexamethasone or TNFα were determined by radioimmunoassay. Results: There was a significant negative correlation between adiponectin gene expression and BMI in subcutaneous adipocytes from 32 women (r = 0.420; p = 0.02). Adiponectin mRNA was also significantly correlated with serum adiponectin (r = 0.44; p = 0.03; n = 25). There was no correlation between adiponectin mRNA expression and BMI in omental adipocytes from 29 women. Leptin mRNA was significantly and positively correlated (r = 0.484; p = 0.01) with BMI in the same omental adipocyte mRNA preparations. In subcutaneous adipocytes from lean subjects, TNFα inhibited adiponectin release by 7.4 ± 1.2% (n = 9, p < 0.05) but had no effect on adiponectin release from subcutaneous or omental adipocytes from obese subjects. Dexamethasone significantly inhibited adiponectin release with 24 hours of treatment. Discussion: The data suggest that reduced adiponectin synthesis in subcutaneous adipocytes contributes to lower serum adiponectin levels in obesity and that glucocorticoids regulate adiponectin gene expression in human adipocytes. TNFα does not seem to directly inhibit adiponectin synthesis in human adipocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 662-669 |
| Number of pages | 8 |
| Journal | Obesity Research |
| Volume | 13 |
| Issue number | 4 |
| State | Published - 2005 |
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Keywords
- Adiponectin
- Human adipocytes
- Tumor necrosis factor α
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Public Health, Environmental and Occupational Health
- Endocrinology
- Food Science
- Endocrinology, Diabetes and Metabolism
Cite this
Regulation of adiponectin expression in human adipocytes : Effects of adiposity, glucocorticoids, and tumor necrosis factor α. / Degawa-Yamauchi, Mikako; Moss, Katherine A.; Bovenkerk, Jason E.; Shankar, Sudha S.; Morrison, Charles L.; Lelliott, Christopher J.; Vidal-Puig, Antonio; Jones, RoseMarie; Considine, Robert.
In: Obesity Research, Vol. 13, No. 4, 2005, p. 662-669.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of adiponectin expression in human adipocytes
T2 - Effects of adiposity, glucocorticoids, and tumor necrosis factor α
AU - Degawa-Yamauchi, Mikako
AU - Moss, Katherine A.
AU - Bovenkerk, Jason E.
AU - Shankar, Sudha S.
AU - Morrison, Charles L.
AU - Lelliott, Christopher J.
AU - Vidal-Puig, Antonio
AU - Jones, RoseMarie
AU - Considine, Robert
PY - 2005
Y1 - 2005
N2 - Objective: Adiponectin mRNA expression in isolated subcutaneous and omental adipocytes was examined across a wide range of adiposity to determine whether adiponectin synthesis is impaired in these adipose tissue depots in obese humans. Tumor necrosis factor (TNF)α and dexamethasone were tested for inhibitory effects on adiponectin release from human adipocytes in vitro. Research Methods and Procedures: Adipocytes were isolated by collagenase digestion of abdominal adipose tissue obtained from subjects undergoing surgical procedures or outpatient needle biopsy. Adiponectin and leptin mRNA were quantitated by real-time reverse transcriptase-polymerase chain reaction. Adiponectin and leptin secretion from isolated adipocytes treated with dexamethasone or TNFα were determined by radioimmunoassay. Results: There was a significant negative correlation between adiponectin gene expression and BMI in subcutaneous adipocytes from 32 women (r = 0.420; p = 0.02). Adiponectin mRNA was also significantly correlated with serum adiponectin (r = 0.44; p = 0.03; n = 25). There was no correlation between adiponectin mRNA expression and BMI in omental adipocytes from 29 women. Leptin mRNA was significantly and positively correlated (r = 0.484; p = 0.01) with BMI in the same omental adipocyte mRNA preparations. In subcutaneous adipocytes from lean subjects, TNFα inhibited adiponectin release by 7.4 ± 1.2% (n = 9, p < 0.05) but had no effect on adiponectin release from subcutaneous or omental adipocytes from obese subjects. Dexamethasone significantly inhibited adiponectin release with 24 hours of treatment. Discussion: The data suggest that reduced adiponectin synthesis in subcutaneous adipocytes contributes to lower serum adiponectin levels in obesity and that glucocorticoids regulate adiponectin gene expression in human adipocytes. TNFα does not seem to directly inhibit adiponectin synthesis in human adipocytes.
AB - Objective: Adiponectin mRNA expression in isolated subcutaneous and omental adipocytes was examined across a wide range of adiposity to determine whether adiponectin synthesis is impaired in these adipose tissue depots in obese humans. Tumor necrosis factor (TNF)α and dexamethasone were tested for inhibitory effects on adiponectin release from human adipocytes in vitro. Research Methods and Procedures: Adipocytes were isolated by collagenase digestion of abdominal adipose tissue obtained from subjects undergoing surgical procedures or outpatient needle biopsy. Adiponectin and leptin mRNA were quantitated by real-time reverse transcriptase-polymerase chain reaction. Adiponectin and leptin secretion from isolated adipocytes treated with dexamethasone or TNFα were determined by radioimmunoassay. Results: There was a significant negative correlation between adiponectin gene expression and BMI in subcutaneous adipocytes from 32 women (r = 0.420; p = 0.02). Adiponectin mRNA was also significantly correlated with serum adiponectin (r = 0.44; p = 0.03; n = 25). There was no correlation between adiponectin mRNA expression and BMI in omental adipocytes from 29 women. Leptin mRNA was significantly and positively correlated (r = 0.484; p = 0.01) with BMI in the same omental adipocyte mRNA preparations. In subcutaneous adipocytes from lean subjects, TNFα inhibited adiponectin release by 7.4 ± 1.2% (n = 9, p < 0.05) but had no effect on adiponectin release from subcutaneous or omental adipocytes from obese subjects. Dexamethasone significantly inhibited adiponectin release with 24 hours of treatment. Discussion: The data suggest that reduced adiponectin synthesis in subcutaneous adipocytes contributes to lower serum adiponectin levels in obesity and that glucocorticoids regulate adiponectin gene expression in human adipocytes. TNFα does not seem to directly inhibit adiponectin synthesis in human adipocytes.
KW - Adiponectin
KW - Human adipocytes
KW - Tumor necrosis factor α
UR - http://www.scopus.com/inward/record.url?scp=24044451713&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24044451713&partnerID=8YFLogxK
M3 - Article
C2 - 15897474
AN - SCOPUS:24044451713
VL - 13
SP - 662
EP - 669
JO - Obesity
JF - Obesity
SN - 1930-7381
IS - 4
ER -