Regulation of anxiety by GABAA receptors in the rat amygdala

S. K. Sanders, Anantha Shekhar

Research output: Contribution to journalArticle

220 Citations (Scopus)

Abstract

Blockade of GABAergic inhibition in the region of the anterior basolateral amygdala (BLA) of rats elicits physiologic changes associated with a defense reaction. The present study was undertaken to determine whether GABA receptors in the BLA might be involved in regulating experimental anxiety using the social interaction (SI) and conflict test. Guide cannulae were stereotaxically implanted bilaterally in the BLA of rats for intracerebral microinjections. In the BLA, injection of the GABAA receptor antagonists bicuculline methiodide (BMI) and picrotoxin (PIC) produced anxiogenic-like effects in the SI paradigm, as did BMI injection using the conflict paradigm. Injection of the GABAA agonist muscimol (MUS) into the central nucleus of the amygdala (Ce) produced anxiolytic-like effects in the SI test. Microinjection of MUS, baclofen (GABAB agonist), 2OH-saclofen (GABAB antagonist) or strychnine (glycine antagonist) into the BLA or BMI into the Ce elicited no change in experimental anxiety as measured by the SI test. These results suggest that endogenous GABA acts tonically at GABAA receptors in the BLA to inhibit anxiety responses.

Original languageEnglish
Pages (from-to)701-706
Number of pages6
JournalPharmacology Biochemistry and Behavior
Volume52
Issue number4
DOIs
StatePublished - 1995

Fingerprint

GABA-A Receptors
Amygdala
Rats
Muscimol
Anxiety
Interpersonal Relations
GABA-A Receptor Agonists
GABA-A Receptor Antagonists
Strychnine
Picrotoxin
Baclofen
GABA Receptors
Anti-Anxiety Agents
Microinjections
Glycine
gamma-Aminobutyric Acid
Injections
Basolateral Nuclear Complex
bicuculline methiodide

Keywords

  • Anxiety conflict
  • Bicuculline
  • Muscimol
  • Picrotoxin
  • Social interaction

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Behavioral Neuroscience
  • Biological Psychiatry
  • Pharmacology
  • Toxicology

Cite this

Regulation of anxiety by GABAA receptors in the rat amygdala. / Sanders, S. K.; Shekhar, Anantha.

In: Pharmacology Biochemistry and Behavior, Vol. 52, No. 4, 1995, p. 701-706.

Research output: Contribution to journalArticle

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