Regulation of branched-chain amino acid catabolism

Robert Harris, K. M. Popov, Y. Zhao, Y. Shimomura

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Catabolism of the branched-chain amino acids is regulated in part at the step catalyzed by the branched-chain α-ketoacid dehydrogenase complex. Previous work suggests both short-term and long-term control mechanisms are involved in regulation of the kinase responsible for phosphorylation and inactivation of the branched-chain α-ketoacid dehydrogenase complex. Recent work of this laboratory has focused on the isolation, characterization and molecular cloning of the branched-chain α-ketoacid dehydrogenase kinase. The cDNA obtained encodes the complete mature protein of 412 amino acids along with a mitochondrial entry sequence of 30 amino acids. Analysis of the deduced amino acid sequence revealed little similarity with eukaryotic Ser/Thr protein kinases. However, the kinase shows considerable sequence similarity with prokaryotic histidine protein kinases. The availability of this cDNA will facilitate gene expression studies of this important regulatory enzyme for the branched-chain α-ketoacid dehydrogenase complex.

Original languageEnglish
JournalJournal of Nutrition
Volume124
Issue number8 SUPPL.
StatePublished - 1994

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3-methyl-2-oxobutanoate dehydrogenase (lipoamide)
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Branched Chain Amino Acids
branched chain amino acids
amino acid metabolism
phosphotransferases (kinases)
Phosphotransferases
protein-histidine kinases
Complementary DNA
amino acids
Protein Sequence Analysis
Molecular Cloning
protein kinases
Protein Kinases
molecular cloning
Amino Acid Sequence
phosphorylation
inactivation
amino acid sequences
Phosphorylation

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Harris, R., Popov, K. M., Zhao, Y., & Shimomura, Y. (1994). Regulation of branched-chain amino acid catabolism. Journal of Nutrition, 124(8 SUPPL.).

Regulation of branched-chain amino acid catabolism. / Harris, Robert; Popov, K. M.; Zhao, Y.; Shimomura, Y.

In: Journal of Nutrition, Vol. 124, No. 8 SUPPL., 1994.

Research output: Contribution to journalArticle

Harris, R, Popov, KM, Zhao, Y & Shimomura, Y 1994, 'Regulation of branched-chain amino acid catabolism', Journal of Nutrition, vol. 124, no. 8 SUPPL..
Harris R, Popov KM, Zhao Y, Shimomura Y. Regulation of branched-chain amino acid catabolism. Journal of Nutrition. 1994;124(8 SUPPL.).
Harris, Robert ; Popov, K. M. ; Zhao, Y. ; Shimomura, Y. / Regulation of branched-chain amino acid catabolism. In: Journal of Nutrition. 1994 ; Vol. 124, No. 8 SUPPL.
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