Regulation of cell movement is mediated by stretch-activated calcium channels

Jullet Lee, Akira Ishihara, Gerry Oxford, Barry Johnson, Ken Jacobson

Research output: Contribution to journalArticle

290 Citations (Scopus)

Abstract

Intracellular calcium regulates many of the molecular processes that are essential for cell movement. It is required for the production of actomyosin- based contractile forces, the regulation of the structure and dynamics of the actin cytoskeleton, and the formation and disassembly of cell-substratum adhesions. Calcium also serves as a second messenger in many biochemical signal-transduction pathways. However, despite the pivotal role of calcium in motile processes, it is not clear how calcium regulates overall cell movement. Here we show that transient increases in intracellular calcium, [Ca2+](i), during the locomotion of fish epithelial keratocytes, occur more frequently in cells that become temporarily 'stuck' to the substratum or when subjected to mechanical stretching. We find that calcium transients arise from the activation of stretch-activated calcium channels, which triggers an influx of extracellular calcium. In addition, the subsequent increase in [Ca2+](i), during the locomotion cell margin. Thus, we have defined a mechanism by which cells can detect and transduce mechanical forces into biochemical signals that can modulate locomotion.

Original languageEnglish (US)
Pages (from-to)382-386
Number of pages5
JournalNature
Volume400
Issue number6742
DOIs
StatePublished - Jul 22 1999
Externally publishedYes

Fingerprint

Calcium Channels
Cell Movement
Calcium
Locomotion
Actomyosin
Second Messenger Systems
Actin Cytoskeleton
Cell Adhesion
Signal Transduction
Fishes

ASJC Scopus subject areas

  • General

Cite this

Lee, J., Ishihara, A., Oxford, G., Johnson, B., & Jacobson, K. (1999). Regulation of cell movement is mediated by stretch-activated calcium channels. Nature, 400(6742), 382-386. https://doi.org/10.1038/22578

Regulation of cell movement is mediated by stretch-activated calcium channels. / Lee, Jullet; Ishihara, Akira; Oxford, Gerry; Johnson, Barry; Jacobson, Ken.

In: Nature, Vol. 400, No. 6742, 22.07.1999, p. 382-386.

Research output: Contribution to journalArticle

Lee, J, Ishihara, A, Oxford, G, Johnson, B & Jacobson, K 1999, 'Regulation of cell movement is mediated by stretch-activated calcium channels', Nature, vol. 400, no. 6742, pp. 382-386. https://doi.org/10.1038/22578
Lee J, Ishihara A, Oxford G, Johnson B, Jacobson K. Regulation of cell movement is mediated by stretch-activated calcium channels. Nature. 1999 Jul 22;400(6742):382-386. https://doi.org/10.1038/22578
Lee, Jullet ; Ishihara, Akira ; Oxford, Gerry ; Johnson, Barry ; Jacobson, Ken. / Regulation of cell movement is mediated by stretch-activated calcium channels. In: Nature. 1999 ; Vol. 400, No. 6742. pp. 382-386.
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