Regulation of diacylglycerol kinase biosynthesis in Escherichia coli. A trans-acting dgkR mutation increases transcription of the structural gene

J. P. Walsh, C. R. Loomis, R. M. Bell

Research output: Contribution to journalArticle

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Abstract

The mechanism of a trans-acting mutation, dgkR1, which causes a 7-fold elevation of diacylglycerol kinase activity in membranes was investigated by direct measurement of diacylglycerol kinase polypeptide was high performance liquid chromatography and by construction of fusions of the dgkA promoter to β-galactosidase and galactokinase. The dgkR1 mutation was demonstrated to act by increasing the transcription of the structural gene for diacylglycerol kinase, dgkA. Additionally, sn-glycerol-3-phosphate acyltransferase activities were shown to be decreased 30-50% in membranes from dkgR1 mutant strains. Increased diacylglycerol levels occurred when cells were grown on low osmolarity media. This did not affect dkgA expression. In a dgkR+ background, enhanced expression of sn-1,2-diacylglycerol kinase activity in cells containing a high copy number plasmid bearing dgkA decreased sn-1,2-diacylglycerol levels. However, overproduction of diacylglycerol kinase in a dgkR1 genetic background did not affect diacylglycerol levels, suggesting that the dgkR1 mutation affects diacylglycerol metabolism by mechanisms additional to enhancement of dgkA transcription.

Original languageEnglish (US)
Pages (from-to)11021-11027
Number of pages7
JournalJournal of Biological Chemistry
Volume261
Issue number24
StatePublished - 1986
Externally publishedYes

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Diacylglycerol Kinase
Biosynthesis
Transcription
Escherichia coli
Genes
Diglycerides
Mutation
Bearings (structural)
Galactokinase
Galactosidases
Membranes
Acyltransferases
High performance liquid chromatography
Metabolism
Osmolar Concentration
Plasmids
Fusion reactions
High Pressure Liquid Chromatography
Peptides

ASJC Scopus subject areas

  • Biochemistry

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Regulation of diacylglycerol kinase biosynthesis in Escherichia coli. A trans-acting dgkR mutation increases transcription of the structural gene. / Walsh, J. P.; Loomis, C. R.; Bell, R. M.

In: Journal of Biological Chemistry, Vol. 261, No. 24, 1986, p. 11021-11027.

Research output: Contribution to journalArticle

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