Regulation of early human hematopoietic (BFU-E and CFU-GEMM) progenitor cells in vitro by interleukin 1-induced fibroblast-conditioned medium

J. R. Zucali, Hal Broxmeyer, C. A. Dinarello, M. A. Gross, R. S. Weiner

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Stimulators of human erythroid burst-forming units (BFU-E) and multipotential colony-forming cells (CFU-GEMM) can be produced by a number of different cell types. A product of human peripheral blood monocytes, interleukin 1 (IL-1), was evaluated for its ability to stimulate fibroblast cultures to produce stimulators of human bone marrow BFU-E and CFU-GEMM colony formation. BFU-E and CFU-GEMM colony formation was evaluated using low-density, nonadherent low-density, and T lymphocyte-depleted nonadherent low-density human bone marrow cells cultured in the presence of a source of pure human erythropoietin. Both human monocyte conditioned medium (MCM) and human recombinant IL-1 (hrIL-1) induced fibroblasts to produce stimulators of BFU-E and CFU-GEMM in a dose-dependent fashion with maximal colony formation occurring when fibroblasts were stimulated by 25% MCM or 140 ng/mL ROO6B hrIL-1, or 1.25 to 5 ng/mL ROO6T hrIL-1. Preincubation of MCM and hrIL-1 with an antibody to IL-1 inactivated the ability of MCM and hrIL-1 to induce the release of erythroid and multipotential colony stimulating activity from fibroblasts. These results suggest that monocyte-derived IL-1 is involved in regulating the production of humoral stimulators of early human hematopoietic progenitors.

Original languageEnglish (US)
Pages (from-to)33-37
Number of pages5
JournalBlood
Volume69
Issue number1
StatePublished - 1987
Externally publishedYes

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Myeloid Progenitor Cells
Erythroid Precursor Cells
Fibroblasts
Conditioned Culture Medium
Interleukin-1
Stem Cells
Monocytes
Bone
T-cells
In Vitro Techniques
Erythropoietin
Cell culture
Blood
Cells
Bone Marrow Cells
Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Regulation of early human hematopoietic (BFU-E and CFU-GEMM) progenitor cells in vitro by interleukin 1-induced fibroblast-conditioned medium. / Zucali, J. R.; Broxmeyer, Hal; Dinarello, C. A.; Gross, M. A.; Weiner, R. S.

In: Blood, Vol. 69, No. 1, 1987, p. 33-37.

Research output: Contribution to journalArticle

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T1 - Regulation of early human hematopoietic (BFU-E and CFU-GEMM) progenitor cells in vitro by interleukin 1-induced fibroblast-conditioned medium

AU - Zucali, J. R.

AU - Broxmeyer, Hal

AU - Dinarello, C. A.

AU - Gross, M. A.

AU - Weiner, R. S.

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