Regulation of egr-1 gene expression by retinoic acid in a human growth factor-dependent cell line

Masato Horie, Kathleen M. Sakamoto, Hal E. Broxmeyer

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Retinoic acid (RA) has profound suppressive effects on growth and survival of human growth factor-dependent cell line, M07e. Treatment of M07e cells by RA reduced expression of egr-1 gene, while the levels of c-myc gene expression remained similar. Suppression of egr-1 gene expression by RA was dosage-dependent and reached maximum at 4 h after RA addition. The decay of egr-1 mRNA was similar in M07e cells treated with or without RA. The transcriptional activity of the promoter region up to -600 or -480 bp upstream of the egr-1 gene was greatly reduced by RA treatment. These data suggest that biological effects of RA on hematopoietic cells may, in part, be mediated by transcriptional suppression of egr-1 gene through its promoter region within -480 bp.

Original languageEnglish (US)
Pages (from-to)303-309
Number of pages7
JournalInternational Journal of Hematology
Volume63
Issue number4
DOIs
StatePublished - Jun 1996

Keywords

  • Cell proliferation
  • Egr-1
  • Gene expression
  • Growth factors
  • Retinoic acid

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Regulation of egr-1 gene expression by retinoic acid in a human growth factor-dependent cell line'. Together they form a unique fingerprint.

  • Cite this