Regulation of egr-1 gene expression by retinoic acid in a human growth factor-dependent cell line

Masato Horie, Kathleen M. Sakamoto, Hal Broxmeyer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Retinoic acid (RA) has profound suppressive effects on growth and survival of human growth factor-dependent cell line, M07e. Treatment of M07e cells by RA reduced expression of egr-1 gene, while the levels of c-myc gene expression remained similar. Suppression of egr-1 gene expression by RA was dosage-dependent and reached maximum at 4 h after RA addition. The decay of egr-1 mRNA was similar in M07e cells treated with or without RA. The transcriptional activity of the promoter region up to -600 or -480 bp upstream of the egr-1 gene was greatly reduced by RA treatment. These data suggest that biological effects of RA on hematopoietic cells may, in part, be mediated by transcriptional suppression of egr-1 gene through its promoter region within -480 bp.

Original languageEnglish
Pages (from-to)303-309
Number of pages7
JournalInternational Journal of Hematology
Volume63
Issue number4
StatePublished - Jun 1996

Fingerprint

Tretinoin
Intercellular Signaling Peptides and Proteins
Gene Expression
Cell Line
Genetic Promoter Regions
Genes
myc Genes
Messenger RNA
Growth

Keywords

  • Cell proliferation
  • Egr-1
  • Gene expression
  • Growth factors
  • Retinoic acid

ASJC Scopus subject areas

  • Hematology

Cite this

Regulation of egr-1 gene expression by retinoic acid in a human growth factor-dependent cell line. / Horie, Masato; Sakamoto, Kathleen M.; Broxmeyer, Hal.

In: International Journal of Hematology, Vol. 63, No. 4, 06.1996, p. 303-309.

Research output: Contribution to journalArticle

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