Regulation of human myelopoiesis by prostaglandin E and lactoferrin

Louis Pelus, Hal Broxmeyer, M. A S Moore

Research output: Contribution to journalArticle

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Abstract

Studies on human myeloid stem cell proliferation indicate that progenitor cell populations committed to monocytoid differentiation are preferentially inhibited by prostaglandin E (PGE). The addition of PGE but not PGF to day 7 CFU(GM) cultures upon initiation results in the dose-dependent inhibition of total colony and cluster formation. Morphological analysis of proliferating clones demonstrates that the effect of PGE on total colony and cluster formation results from the selective inhibition of monocyte-macrophage colony forming cells. Mixed monocytoid/neutrophil colony formation was markedly less sensitive and neutrophil and eosinophil colony formation essentially insensitive to the inhibitory effects of PGE. The inhibition of monocytoid colony formation by PGE1 extends equally well to day 13 CFU(GM), but not to CFU(GM) in suspension culture. The observed effects of PGE1 on monocytoid committed pre-CFU(GM) and day 7 and day 14 CFU(GM) indicate that sensitivity to inhibition by PGE increases with progenitor cell maturity. Specificity analysis indicates that prostaglandins of the E series (PGE1, PGE2) are far the most active naturally occurring prostanoate compounds inhibiting CFU(GM) proliferation. The addition of iron saturated lactoferrin to liquid cultures of human peripheral blood monocytes, inclusion into mononuclear cell feeder layers or addition to agar cultures proliferating in response to endogenously produced CSFs, results in the equivalent inhibition of CSFs necessary for day 7 and day 14 monocytoid and/or neutrophil and eosinophil colony and cluster formation. These results indicate roles for PGE and lactoferrin in myeloid stem cell regulation in vitro and suggest that they may serve as physiological regulators of granulocyte and monocyte proliferation.

Original languageEnglish (US)
Pages (from-to)515-526
Number of pages12
JournalCell and Tissue Kinetics
Volume14
Issue number5
StatePublished - 1981
Externally publishedYes

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Myelopoiesis
Lactoferrin
Granulocyte-Macrophage Progenitor Cells
Prostaglandins E
Alprostadil
Myeloid Progenitor Cells
Monocytes
Neutrophils
Eosinophils
Stem Cells
Feeder Cells
Prostaglandins F
Dinoprostone
Granulocytes
Agar
Suspensions
Iron
Clone Cells
Macrophages
Cell Proliferation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Regulation of human myelopoiesis by prostaglandin E and lactoferrin. / Pelus, Louis; Broxmeyer, Hal; Moore, M. A S.

In: Cell and Tissue Kinetics, Vol. 14, No. 5, 1981, p. 515-526.

Research output: Contribution to journalArticle

Pelus, Louis ; Broxmeyer, Hal ; Moore, M. A S. / Regulation of human myelopoiesis by prostaglandin E and lactoferrin. In: Cell and Tissue Kinetics. 1981 ; Vol. 14, No. 5. pp. 515-526.
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