Regulation of insulin-like growth factor binding protein secretion by a murine mammary epithelial cell line

Todd Skaar, Craig R. Baumrucker

Research output: Contribution to journalArticle

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Abstract

The regulation of insulin-like growth factor binding protein (IGFBP) secretion by mammogenic and lactogenic hormones was investigated in a mouse mammary epithelial cell line (COMMA-D/MME). Cells were grown to confluency on plastic culture plates in serum-containing medium. The confluent cells were exposed to the hormonal treatments in serum-free medium for 6 days. Conditioned medium was collected on Days 5 and 6 and analyzed for IGFBPs by 125I-labeled insulin-like growth factor (IGF)-II ligand blotting and quantified by densitometry. IGFBP data were expressed as absorbance units X millimeters (AUxmm) that were corrected for DNA per well. In basal serum-free media, the COMMA-D/MME cells secreted predominately IGFBP-3, but also some IGFBP-2. The mammogenic growth factors, IGF-I (13.3 nM) and epidermal growth factor (EGF; 1.7 nM), both stimulated DNA synthesis (P <0.05); however, their effects on IGFBP-3 secretion differed. IGF-I stimulated IGFBP-3 secretion whereas EGF was inhibitory. In addition, EGF inhibited IGFBP-2 secretion and IGF-I tended to increase it. No interaction was observed between IGF-I and EGF for any of the parameters measured. Three lactogenic hormones (insulin, 154 nM; prolactin, 4.3 nM; and cortisol, 1.4 μM) in all combinations were tested to determine their effects on IGFBP secretion. Insulin stimulated IGFBP-3 secretion and DNA synthesis, but had no effect on IGFBP-2 secretion. Cortisol inhibited IGFBP-3 secretion and DNA synthesis, but increased IGFBP-2 secretion. Prolactin had no effect on any of the parameters examined. In summary, the COMMA-D/MME cells secrete IGFBP-2 and IGFBP-3 in serum-free media. Although the secretion of IGFBP-2 and IGFBP-3 was regulated by several of the hormones and growth factors tested, no clear distinction was observed between the mammogenic and lactogenic treatments.

Original languageEnglish (US)
Pages (from-to)183-188
Number of pages6
JournalExperimental Cell Research
Volume209
Issue number2
StatePublished - 1993
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor Binding Protein 2
Breast
Epithelial Cells
Epidermal Growth Factor
Cell Line
Insulin-Like Growth Factor I
Serum-Free Culture Media
DNA
Hormones
Prolactin
Hydrocortisone
Intercellular Signaling Peptides and Proteins
Insulin-Like Growth Factor Binding Protein 6
Insulin
Insulin-Like Growth Factor II
Densitometry
Conditioned Culture Medium
Plastics

ASJC Scopus subject areas

  • Cell Biology

Cite this

Regulation of insulin-like growth factor binding protein secretion by a murine mammary epithelial cell line. / Skaar, Todd; Baumrucker, Craig R.

In: Experimental Cell Research, Vol. 209, No. 2, 1993, p. 183-188.

Research output: Contribution to journalArticle

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