Regulation of kipl degradation by phosphorylation

Qi Vjang, Maureen Harrinaton, Mark Goebl

Research output: Contribution to journalArticle

Abstract

The cyclin dependent kinases(CDKs) are critical for progression through the cell cycle. The eel1 cycle protein KIPl, or p27, is an inhibitor of many of the CDK/cyclin complexes. Previously, the KIPl protein has been shown to be ubiquitinated in vivo and in vitro experiments have suggested that this modification requires the Cdc34 ubiquitination enzyme. In yeast , substrates of Cdc34 are believed to be phosphorylated by a CDK complex in order to be recognized by the Cdc34 machinery. In order to determine whether a similar mechanism may function in higher cells, we mutated the potential CDK sites in p27 and assayed the mutant proteins for changes in stability. We identified one mutant which was 5 times more stable than wild-type KIP1 protein. This mutant protein retained its ability to bind CDK2 and inhibit CDK2 kinase activity. These results suggest that the KIPl protein may be targeted for degradation only after CDK phosphorylation.

Original languageEnglish (US)
Pages (from-to)A988
JournalFASEB Journal
Volume11
Issue number9
StatePublished - Dec 1 1997

Fingerprint

Phosphorylation
Cyclin-Dependent Kinases
cyclin-dependent kinase
phosphorylation
Degradation
degradation
Mutant Proteins
proteins
Proteins
mutants
Cyclins
Ubiquitination
Yeast
cyclins
Machinery
Cell Cycle
Phosphotransferases
Yeasts
Cells
cell cycle

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Vjang, Q., Harrinaton, M., & Goebl, M. (1997). Regulation of kipl degradation by phosphorylation. FASEB Journal, 11(9), A988.

Regulation of kipl degradation by phosphorylation. / Vjang, Qi; Harrinaton, Maureen; Goebl, Mark.

In: FASEB Journal, Vol. 11, No. 9, 01.12.1997, p. A988.

Research output: Contribution to journalArticle

Vjang, Q, Harrinaton, M & Goebl, M 1997, 'Regulation of kipl degradation by phosphorylation', FASEB Journal, vol. 11, no. 9, pp. A988.
Vjang Q, Harrinaton M, Goebl M. Regulation of kipl degradation by phosphorylation. FASEB Journal. 1997 Dec 1;11(9):A988.
Vjang, Qi ; Harrinaton, Maureen ; Goebl, Mark. / Regulation of kipl degradation by phosphorylation. In: FASEB Journal. 1997 ; Vol. 11, No. 9. pp. A988.
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