Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages

Maria L. Fiani, Jill Beitz, Diane Turvy, Janice Blum, Philip D. Stahl

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The mannose receptor, present on the plasma membrane of macrophages, promotes the internalization of glycoproteins and glycoconjugates via both endocytic and phagocytic pathways. The expression of this receptor is tightly modulated during monocyte/MΦ differentiation and cellular activation. We isolated clonal populations from murine J774 macrophage tumor cells, which differ in their surface expression of functional mannose receptors. To examine the potential mechanisms regulating receptor function in these cell lines, the interaction of receptor with ligand as well as receptor synthesis and degradation was analyzed. J774 clones with both high and low levels of mannose receptor activity were found to synthesize significant amounts of receptor protein, suggesting that the protein may be regulated at the level of synthesis and degradation. In J774 clones expressing very low receptor activity and protein, the half-life of mannose receptor molecules was substantially decreased. The evolution of multiple mechanisms modulating mannose receptor function may be critical in fine-tuning the role of this receptor in antigen processing and in scavenger and host defense functions.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalJournal of Leukocyte Biology
Volume64
Issue number1
StatePublished - Jul 1998

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Macrophages
Clone Cells
Proteins
Glycoconjugates
Antigen Presentation
Cell Communication
Half-Life
Monocytes
Glycoproteins
Cell Membrane
mannose receptor
Ligands
Cell Line
Population
Neoplasms

Keywords

  • Glycoconjugates
  • Glycoproteins
  • Receptor regulation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages. / Fiani, Maria L.; Beitz, Jill; Turvy, Diane; Blum, Janice; Stahl, Philip D.

In: Journal of Leukocyte Biology, Vol. 64, No. 1, 07.1998, p. 85-91.

Research output: Contribution to journalArticle

Fiani, ML, Beitz, J, Turvy, D, Blum, J & Stahl, PD 1998, 'Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages', Journal of Leukocyte Biology, vol. 64, no. 1, pp. 85-91.
Fiani, Maria L. ; Beitz, Jill ; Turvy, Diane ; Blum, Janice ; Stahl, Philip D. / Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages. In: Journal of Leukocyte Biology. 1998 ; Vol. 64, No. 1. pp. 85-91.
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