Abstract
The mannose receptor, present on the plasma membrane of macrophages, promotes the internalization of glycoproteins and glycoconjugates via both endocytic and phagocytic pathways. The expression of this receptor is tightly modulated during monocyte/MΦ differentiation and cellular activation. We isolated clonal populations from murine J774 macrophage tumor cells, which differ in their surface expression of functional mannose receptors. To examine the potential mechanisms regulating receptor function in these cell lines, the interaction of receptor with ligand as well as receptor synthesis and degradation was analyzed. J774 clones with both high and low levels of mannose receptor activity were found to synthesize significant amounts of receptor protein, suggesting that the protein may be regulated at the level of synthesis and degradation. In J774 clones expressing very low receptor activity and protein, the half-life of mannose receptor molecules was substantially decreased. The evolution of multiple mechanisms modulating mannose receptor function may be critical in fine-tuning the role of this receptor in antigen processing and in scavenger and host defense functions.
| Original language | English |
|---|---|
| Pages (from-to) | 85-91 |
| Number of pages | 7 |
| Journal | Journal of Leukocyte Biology |
| Volume | 64 |
| Issue number | 1 |
| State | Published - Jul 1998 |
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Keywords
- Glycoconjugates
- Glycoproteins
- Receptor regulation
ASJC Scopus subject areas
- Cell Biology
Cite this
Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages. / Fiani, Maria L.; Beitz, Jill; Turvy, Diane; Blum, Janice; Stahl, Philip D.
In: Journal of Leukocyte Biology, Vol. 64, No. 1, 07.1998, p. 85-91.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages
AU - Fiani, Maria L.
AU - Beitz, Jill
AU - Turvy, Diane
AU - Blum, Janice
AU - Stahl, Philip D.
PY - 1998/7
Y1 - 1998/7
N2 - The mannose receptor, present on the plasma membrane of macrophages, promotes the internalization of glycoproteins and glycoconjugates via both endocytic and phagocytic pathways. The expression of this receptor is tightly modulated during monocyte/MΦ differentiation and cellular activation. We isolated clonal populations from murine J774 macrophage tumor cells, which differ in their surface expression of functional mannose receptors. To examine the potential mechanisms regulating receptor function in these cell lines, the interaction of receptor with ligand as well as receptor synthesis and degradation was analyzed. J774 clones with both high and low levels of mannose receptor activity were found to synthesize significant amounts of receptor protein, suggesting that the protein may be regulated at the level of synthesis and degradation. In J774 clones expressing very low receptor activity and protein, the half-life of mannose receptor molecules was substantially decreased. The evolution of multiple mechanisms modulating mannose receptor function may be critical in fine-tuning the role of this receptor in antigen processing and in scavenger and host defense functions.
AB - The mannose receptor, present on the plasma membrane of macrophages, promotes the internalization of glycoproteins and glycoconjugates via both endocytic and phagocytic pathways. The expression of this receptor is tightly modulated during monocyte/MΦ differentiation and cellular activation. We isolated clonal populations from murine J774 macrophage tumor cells, which differ in their surface expression of functional mannose receptors. To examine the potential mechanisms regulating receptor function in these cell lines, the interaction of receptor with ligand as well as receptor synthesis and degradation was analyzed. J774 clones with both high and low levels of mannose receptor activity were found to synthesize significant amounts of receptor protein, suggesting that the protein may be regulated at the level of synthesis and degradation. In J774 clones expressing very low receptor activity and protein, the half-life of mannose receptor molecules was substantially decreased. The evolution of multiple mechanisms modulating mannose receptor function may be critical in fine-tuning the role of this receptor in antigen processing and in scavenger and host defense functions.
KW - Glycoconjugates
KW - Glycoproteins
KW - Receptor regulation
UR - http://www.scopus.com/inward/record.url?scp=0031745275&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031745275&partnerID=8YFLogxK
M3 - Article
VL - 64
SP - 85
EP - 91
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 1
ER -