Regulation of matrix vesicle metabolism by vitamin d metabolites

Barbara D. Boyan, Zvi Schwartz, Larry D. Swain, Lynda Bonewald, Aruna Khare

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Matrix vesicles are membrane organelles found in the extracellular matrix of calcifying cells. Vitamin D-responsive alkaline phosphatase specific activity has been localized to matrix vesicles in chondrocyte and osteoblast cultures. The effect of hormone is both metabolite and cell specific. Alkaline phosphatase in matrix vesicles produced by resting zone chondrocytes is stimulated by 24, 25(OH)2D3 whereas alkaline phosphatase in matrix vesicles produced by growth zone chondrocytes is responsive to 1,25(OH)2D3. However, mesenchymal cell cultures, which exhibit a chondrogenic phenotype when exposed to bone inductive proteins in vitro, produce vesicles with alkaline phosphatase activity that is unaffected by either 1,25(OH)2D3 or 24, 25(OH)2D3. Incorporation and release of arachidonic acid into phosphatidylethanolamine is also differentially regulated by 1,25(OH)2D3 and 24, 25(OH)2D3 in chondrocytes. These data suggest that vitamin D metabolites may regulate endochondral ossification by altering matrix vesicle enzyme activities, perhaps through changes in membrane phospholipid metabolism.

Original languageEnglish (US)
Pages (from-to)629-642
Number of pages14
JournalConnective Tissue Research
Volume22
Issue number1-4
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Chondrocytes
Metabolites
Metabolism
Vitamins
Alkaline Phosphatase
Cell culture
Vitamin D
Membranes
Osteoblasts
Enzyme activity
Arachidonic Acid
Osteogenesis
Organelles
Extracellular Matrix
Phospholipids
Bone
Cell Culture Techniques
Hormones
Phenotype
Bone and Bones

Keywords

  • Acyl transferase
  • Alkaline phosphatase
  • Chondrocyte cultures
  • Matrix vesicles
  • ROS 17/2.8 cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Regulation of matrix vesicle metabolism by vitamin d metabolites. / Boyan, Barbara D.; Schwartz, Zvi; Swain, Larry D.; Bonewald, Lynda; Khare, Aruna.

In: Connective Tissue Research, Vol. 22, No. 1-4, 1989, p. 629-642.

Research output: Contribution to journalArticle

Boyan, Barbara D. ; Schwartz, Zvi ; Swain, Larry D. ; Bonewald, Lynda ; Khare, Aruna. / Regulation of matrix vesicle metabolism by vitamin d metabolites. In: Connective Tissue Research. 1989 ; Vol. 22, No. 1-4. pp. 629-642.
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