Regulation of myelopoiesis by murine fibroblastic and adipogenic cell lines

G. Hangoc, L. R. Daub, R. G. Maze, J. H F Falkenburg, Hal Broxmeyer, Maureen Harrington

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The regulation of hematopoiesis has been suggested to take place in close association with various cell types found in the bone marrow (BM) microenvironment. In the present study the role of fibroblasts, adipocytes and cell surface heparan sulfate in regulating hematopoiesis in an in vitro mouse system was examined. Mouse BM cells were allowed to adhere to a mouse embryo fibroblast cell line (C3H 10T1/2) or a clonally derived adipogenically determined derivative (Clone D) of the 10T1/2 cell line. Nonadherent cells were removed, cultures were overlaid with semisolid media supplemented with growth factors and colony formation by granulocyte-macrophage (CFU-GM), erythroid (BFU-E) and multipotential (CFU-GEMM) progenitor cells was quantitated. Adherence and co-culture of BM cells with the fibroblast cell line resulted in increased numbers of total CFU-GM and CFU-GEMM colonies. In contrast, adherence and co-culture of BM cells with the adipocytic cell line resulted in an increase only in CFU-GEMM colonies. Morphological analysis revealed a preferential adherence/growth of granulocyte and macrophage progenitors at the expense of bipotent granulocyte-macrophage progenitors to the fibroblastic cell line and an increase in the adherence/growth of granulocyte progenitors to the adipogenic cell line. Progenitor cell adherence was abolished when the fibroblastic or adipocytic cell lines were pretreated with heparitinase. These results demonstrate enhanced proliferation/differentiation of hematopoietic progenitor cells when there is direct contact between hematopoietic progenitors and cell types characteristic of those found in the microenvironment and that heparan sulfate and different types of stromal cells appear to play different roles in this interaction.

Original languageEnglish
Pages (from-to)502-507
Number of pages6
JournalExperimental Hematology
Volume21
Issue number4
StatePublished - 1993

Fingerprint

Myelopoiesis
Granulocyte-Macrophage Progenitor Cells
Cell Line
Myeloid Progenitor Cells
Bone Marrow Cells
Heparitin Sulfate
Fibroblasts
Hematopoiesis
heparitinsulfate lyase
Hematopoietic Stem Cells
Coculture Techniques
Stem Cells
Granulocyte Precursor Cells
Erythroid Precursor Cells
Stromal Cells
Growth
Granulocytes
Adipocytes
Intercellular Signaling Peptides and Proteins
Embryonic Structures

Keywords

  • Cell-cell contact
  • Colony formation
  • Microenvironment

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Regulation of myelopoiesis by murine fibroblastic and adipogenic cell lines. / Hangoc, G.; Daub, L. R.; Maze, R. G.; Falkenburg, J. H F; Broxmeyer, Hal; Harrington, Maureen.

In: Experimental Hematology, Vol. 21, No. 4, 1993, p. 502-507.

Research output: Contribution to journalArticle

Hangoc, G, Daub, LR, Maze, RG, Falkenburg, JHF, Broxmeyer, H & Harrington, M 1993, 'Regulation of myelopoiesis by murine fibroblastic and adipogenic cell lines', Experimental Hematology, vol. 21, no. 4, pp. 502-507.
Hangoc, G. ; Daub, L. R. ; Maze, R. G. ; Falkenburg, J. H F ; Broxmeyer, Hal ; Harrington, Maureen. / Regulation of myelopoiesis by murine fibroblastic and adipogenic cell lines. In: Experimental Hematology. 1993 ; Vol. 21, No. 4. pp. 502-507.
@article{7c1354ff369a426c9ed9e573dba8cf0d,
title = "Regulation of myelopoiesis by murine fibroblastic and adipogenic cell lines",
abstract = "The regulation of hematopoiesis has been suggested to take place in close association with various cell types found in the bone marrow (BM) microenvironment. In the present study the role of fibroblasts, adipocytes and cell surface heparan sulfate in regulating hematopoiesis in an in vitro mouse system was examined. Mouse BM cells were allowed to adhere to a mouse embryo fibroblast cell line (C3H 10T1/2) or a clonally derived adipogenically determined derivative (Clone D) of the 10T1/2 cell line. Nonadherent cells were removed, cultures were overlaid with semisolid media supplemented with growth factors and colony formation by granulocyte-macrophage (CFU-GM), erythroid (BFU-E) and multipotential (CFU-GEMM) progenitor cells was quantitated. Adherence and co-culture of BM cells with the fibroblast cell line resulted in increased numbers of total CFU-GM and CFU-GEMM colonies. In contrast, adherence and co-culture of BM cells with the adipocytic cell line resulted in an increase only in CFU-GEMM colonies. Morphological analysis revealed a preferential adherence/growth of granulocyte and macrophage progenitors at the expense of bipotent granulocyte-macrophage progenitors to the fibroblastic cell line and an increase in the adherence/growth of granulocyte progenitors to the adipogenic cell line. Progenitor cell adherence was abolished when the fibroblastic or adipocytic cell lines were pretreated with heparitinase. These results demonstrate enhanced proliferation/differentiation of hematopoietic progenitor cells when there is direct contact between hematopoietic progenitors and cell types characteristic of those found in the microenvironment and that heparan sulfate and different types of stromal cells appear to play different roles in this interaction.",
keywords = "Cell-cell contact, Colony formation, Microenvironment",
author = "G. Hangoc and Daub, {L. R.} and Maze, {R. G.} and Falkenburg, {J. H F} and Hal Broxmeyer and Maureen Harrington",
year = "1993",
language = "English",
volume = "21",
pages = "502--507",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Regulation of myelopoiesis by murine fibroblastic and adipogenic cell lines

AU - Hangoc, G.

AU - Daub, L. R.

AU - Maze, R. G.

AU - Falkenburg, J. H F

AU - Broxmeyer, Hal

AU - Harrington, Maureen

PY - 1993

Y1 - 1993

N2 - The regulation of hematopoiesis has been suggested to take place in close association with various cell types found in the bone marrow (BM) microenvironment. In the present study the role of fibroblasts, adipocytes and cell surface heparan sulfate in regulating hematopoiesis in an in vitro mouse system was examined. Mouse BM cells were allowed to adhere to a mouse embryo fibroblast cell line (C3H 10T1/2) or a clonally derived adipogenically determined derivative (Clone D) of the 10T1/2 cell line. Nonadherent cells were removed, cultures were overlaid with semisolid media supplemented with growth factors and colony formation by granulocyte-macrophage (CFU-GM), erythroid (BFU-E) and multipotential (CFU-GEMM) progenitor cells was quantitated. Adherence and co-culture of BM cells with the fibroblast cell line resulted in increased numbers of total CFU-GM and CFU-GEMM colonies. In contrast, adherence and co-culture of BM cells with the adipocytic cell line resulted in an increase only in CFU-GEMM colonies. Morphological analysis revealed a preferential adherence/growth of granulocyte and macrophage progenitors at the expense of bipotent granulocyte-macrophage progenitors to the fibroblastic cell line and an increase in the adherence/growth of granulocyte progenitors to the adipogenic cell line. Progenitor cell adherence was abolished when the fibroblastic or adipocytic cell lines were pretreated with heparitinase. These results demonstrate enhanced proliferation/differentiation of hematopoietic progenitor cells when there is direct contact between hematopoietic progenitors and cell types characteristic of those found in the microenvironment and that heparan sulfate and different types of stromal cells appear to play different roles in this interaction.

AB - The regulation of hematopoiesis has been suggested to take place in close association with various cell types found in the bone marrow (BM) microenvironment. In the present study the role of fibroblasts, adipocytes and cell surface heparan sulfate in regulating hematopoiesis in an in vitro mouse system was examined. Mouse BM cells were allowed to adhere to a mouse embryo fibroblast cell line (C3H 10T1/2) or a clonally derived adipogenically determined derivative (Clone D) of the 10T1/2 cell line. Nonadherent cells were removed, cultures were overlaid with semisolid media supplemented with growth factors and colony formation by granulocyte-macrophage (CFU-GM), erythroid (BFU-E) and multipotential (CFU-GEMM) progenitor cells was quantitated. Adherence and co-culture of BM cells with the fibroblast cell line resulted in increased numbers of total CFU-GM and CFU-GEMM colonies. In contrast, adherence and co-culture of BM cells with the adipocytic cell line resulted in an increase only in CFU-GEMM colonies. Morphological analysis revealed a preferential adherence/growth of granulocyte and macrophage progenitors at the expense of bipotent granulocyte-macrophage progenitors to the fibroblastic cell line and an increase in the adherence/growth of granulocyte progenitors to the adipogenic cell line. Progenitor cell adherence was abolished when the fibroblastic or adipocytic cell lines were pretreated with heparitinase. These results demonstrate enhanced proliferation/differentiation of hematopoietic progenitor cells when there is direct contact between hematopoietic progenitors and cell types characteristic of those found in the microenvironment and that heparan sulfate and different types of stromal cells appear to play different roles in this interaction.

KW - Cell-cell contact

KW - Colony formation

KW - Microenvironment

UR - http://www.scopus.com/inward/record.url?scp=0027892629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027892629&partnerID=8YFLogxK

M3 - Article

VL - 21

SP - 502

EP - 507

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 4

ER -