Regulation of pancreatic protein synthesis by cholecystokinin and calcium

Murray Korc

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The effect of cholecystokinin (CCK) on [3H]phenylalanine and [14C]tyrosine incorporation into protein was studied in isolated rat pancreatic acini. CCK8, the biologically active octapeptide of CCK, had a biphasic effect on amino acid incorporation. The magnitude of the stimulatory effect (maximal at 10-10 to 3 x 10-10 M CCK8) was greater in diabetic rats, whereas the magnitude of the inhibitory effect (maximal at 10-8 M CCK8) was greater in normal rats. Omission of extracellular Ca2+ from the incubation media did not diminish the stimulatory action of CCK8. Addition of 10-4 M EGTA to media containing no added Ca2+ lowered basal incorporation, abolished CCK's stimulatory effect, and enhanced its inhibitory effect. Stimulation was restored in the presence of Ca2+. The stimulatory effect of the cholinergic analogue carbachol (10-5 M) on amino acid incorporation was also abolished by removal of extracellular Ca2+, whereas the stimulatory effect of insulin (1.67 x 10-8 M) remained intact. These findings suggest that CCK and other pancreatic secretagogues enhanced pancreatic protein synthesis via Ca2+, whereas stimulation by insulin occurs via another mechanism.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume6
Issue number1
StatePublished - 1982
Externally publishedYes

Fingerprint

Cholecystokinin
Calcium
Insulin
Amino Acids
Sincalide
Proteins
Egtazic Acid
Carbachol
Phenylalanine
Cholinergic Agents
Tyrosine

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

@article{d2ddd68b1add4c40979b1783583ed7f0,
title = "Regulation of pancreatic protein synthesis by cholecystokinin and calcium",
abstract = "The effect of cholecystokinin (CCK) on [3H]phenylalanine and [14C]tyrosine incorporation into protein was studied in isolated rat pancreatic acini. CCK8, the biologically active octapeptide of CCK, had a biphasic effect on amino acid incorporation. The magnitude of the stimulatory effect (maximal at 10-10 to 3 x 10-10 M CCK8) was greater in diabetic rats, whereas the magnitude of the inhibitory effect (maximal at 10-8 M CCK8) was greater in normal rats. Omission of extracellular Ca2+ from the incubation media did not diminish the stimulatory action of CCK8. Addition of 10-4 M EGTA to media containing no added Ca2+ lowered basal incorporation, abolished CCK's stimulatory effect, and enhanced its inhibitory effect. Stimulation was restored in the presence of Ca2+. The stimulatory effect of the cholinergic analogue carbachol (10-5 M) on amino acid incorporation was also abolished by removal of extracellular Ca2+, whereas the stimulatory effect of insulin (1.67 x 10-8 M) remained intact. These findings suggest that CCK and other pancreatic secretagogues enhanced pancreatic protein synthesis via Ca2+, whereas stimulation by insulin occurs via another mechanism.",
author = "Murray Korc",
year = "1982",
language = "English (US)",
volume = "6",
journal = "American Journal of Physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - Regulation of pancreatic protein synthesis by cholecystokinin and calcium

AU - Korc, Murray

PY - 1982

Y1 - 1982

N2 - The effect of cholecystokinin (CCK) on [3H]phenylalanine and [14C]tyrosine incorporation into protein was studied in isolated rat pancreatic acini. CCK8, the biologically active octapeptide of CCK, had a biphasic effect on amino acid incorporation. The magnitude of the stimulatory effect (maximal at 10-10 to 3 x 10-10 M CCK8) was greater in diabetic rats, whereas the magnitude of the inhibitory effect (maximal at 10-8 M CCK8) was greater in normal rats. Omission of extracellular Ca2+ from the incubation media did not diminish the stimulatory action of CCK8. Addition of 10-4 M EGTA to media containing no added Ca2+ lowered basal incorporation, abolished CCK's stimulatory effect, and enhanced its inhibitory effect. Stimulation was restored in the presence of Ca2+. The stimulatory effect of the cholinergic analogue carbachol (10-5 M) on amino acid incorporation was also abolished by removal of extracellular Ca2+, whereas the stimulatory effect of insulin (1.67 x 10-8 M) remained intact. These findings suggest that CCK and other pancreatic secretagogues enhanced pancreatic protein synthesis via Ca2+, whereas stimulation by insulin occurs via another mechanism.

AB - The effect of cholecystokinin (CCK) on [3H]phenylalanine and [14C]tyrosine incorporation into protein was studied in isolated rat pancreatic acini. CCK8, the biologically active octapeptide of CCK, had a biphasic effect on amino acid incorporation. The magnitude of the stimulatory effect (maximal at 10-10 to 3 x 10-10 M CCK8) was greater in diabetic rats, whereas the magnitude of the inhibitory effect (maximal at 10-8 M CCK8) was greater in normal rats. Omission of extracellular Ca2+ from the incubation media did not diminish the stimulatory action of CCK8. Addition of 10-4 M EGTA to media containing no added Ca2+ lowered basal incorporation, abolished CCK's stimulatory effect, and enhanced its inhibitory effect. Stimulation was restored in the presence of Ca2+. The stimulatory effect of the cholinergic analogue carbachol (10-5 M) on amino acid incorporation was also abolished by removal of extracellular Ca2+, whereas the stimulatory effect of insulin (1.67 x 10-8 M) remained intact. These findings suggest that CCK and other pancreatic secretagogues enhanced pancreatic protein synthesis via Ca2+, whereas stimulation by insulin occurs via another mechanism.

UR - http://www.scopus.com/inward/record.url?scp=0019952478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019952478&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0019952478

VL - 6

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1857

IS - 1

ER -