Regulation of phospholipase d and its role in membrane traffic

P. C. Sternweis, H. A. Brown, W. D. Singer, X. Jiang, A. Faulkner, Clark Wells, M. G. Roth, N. T. Ktistakis, K. Bi

Research output: Contribution to journalArticle

Abstract

Phospholipase D (PLD) converts phospholipids into phosphatidic acid (PA). A mammalian form of the enzyme which hydrolyzes phosphatidylcholine preferentially is regulated by two classes of monomeric G proteins, Arf and Rho, and by protein kinase C (PKC). All three classes of regulators require activation and their stimulatory effects on PLD are synergistic, in vitro. The action of PKC occurs through its regulatory domain rather than through phosphorylation by its kinase activity. Arf-sensitive PLD activity is higher in preparations enriched for Golgi membranes. This Arf-stimulated activity is inhibited by brefeldin A in membranes of cells sensitive to this toxin. In the presence of ethanol, PLD forms phosphatidylethanol rather than phosphatidic acid. Ethanol inhibited Arf-dependent formation of coated vesicfes in an in vitro system. Treatment of Golgi membranes with an exogenous PLD enzyme stimulated binding of coatomer to these membranes. A phosphatase activity that hydrolyzes PA is also enriched in Golgi preparations. These and other data indicate that mammalian PLD and production of PA is regulated in a complex fashion by a variety of signalling pathways and suggest that the action of PLD and phospholipid turnover is important in orocesses of intracellular orotein traffic.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997
Externally publishedYes

Fingerprint

Phospholipase D
phospholipase D
Phospholipases
traffic
Phosphatidic Acids
Membranes
acids
protein kinase C
4 alpha-glucanotransferase
Protein Kinase C
Phospholipids
phospholipids
Ethanol
ethanol
brefeldin A
Brefeldin A
rho-Associated Kinases
Phosphorylation
Monomeric GTP-Binding Proteins
enzymes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Sternweis, P. C., Brown, H. A., Singer, W. D., Jiang, X., Faulkner, A., Wells, C., ... Bi, K. (1997). Regulation of phospholipase d and its role in membrane traffic. FASEB Journal, 11(9).

Regulation of phospholipase d and its role in membrane traffic. / Sternweis, P. C.; Brown, H. A.; Singer, W. D.; Jiang, X.; Faulkner, A.; Wells, Clark; Roth, M. G.; Ktistakis, N. T.; Bi, K.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

Sternweis, PC, Brown, HA, Singer, WD, Jiang, X, Faulkner, A, Wells, C, Roth, MG, Ktistakis, NT & Bi, K 1997, 'Regulation of phospholipase d and its role in membrane traffic', FASEB Journal, vol. 11, no. 9.
Sternweis PC, Brown HA, Singer WD, Jiang X, Faulkner A, Wells C et al. Regulation of phospholipase d and its role in membrane traffic. FASEB Journal. 1997;11(9).
Sternweis, P. C. ; Brown, H. A. ; Singer, W. D. ; Jiang, X. ; Faulkner, A. ; Wells, Clark ; Roth, M. G. ; Ktistakis, N. T. ; Bi, K. / Regulation of phospholipase d and its role in membrane traffic. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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