Regulation of the proliferation of the established human monoblast cell line, U937, at the single cell level

E. Berman, B. Y. Rubin, Hal Broxmeyer

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

U937 cells, an established monoblast or early monocyte cell line, were assessed as a model in vitro for the regulation of cell growth at the single cell level. Colony formation by 500 U937 cells, preinduced to a state of responsiveness to lactoferrin (LF) by incubation with human γ interferon was suppressed by LF. LF-suppressed colony formation was restored by partially purified growth activity derived from U937 cells. The release of growth factor(s) into conditioned medium required concentrations of >500 U937 cells/ml and this release was dependent on the length of time that the cells conditioned the culture medium. This release was suppressed by LF. U937 cells were induced to a state of responsiveness to LF by incubation with human γ interferon, washed, and plated as a single cell per well. Individual cells formed colonies with a cloning efficiency of ~50% which equalled the cloning efficiency detected when 500 U937 cells/ml were plated, suggesting that U937 colony forming cells might contain endogenous growth activity. Detection of these endogenous growth activities required the use of LF. The cloning efficiency of individually isolated U937 cells was suppressed by ~50% with LF, similar to the LF suppression of colony formation when 500 cells/ml were plated. That the LF-suppressed U937 colony forming cells required growth activity was suggested as the cloning efficiency of LF-suppressed individually isolated U937 colony forming cells was restored by partially purified U937 growth activity. Partially purified U937 growth activity did not stimulate, enhance, or inhibit colony formation by normal human bone marrow granulocyte-macrophage progenitors. U937 cells can thus serve as a useful model for the study of growth regulation at the level of a single cell.

Original languageEnglish (US)
Pages (from-to)3309-3312
Number of pages4
JournalCancer Research
Volume46
Issue number7
StatePublished - 1986

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Monocyte-Macrophage Precursor Cells
Lactoferrin
U937 Cells
Cell Line
Growth
Organism Cloning
Conditioned Culture Medium
Interferons
Granulocyte-Macrophage Progenitor Cells
Monocytes
Intercellular Signaling Peptides and Proteins
Cell Culture Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Regulation of the proliferation of the established human monoblast cell line, U937, at the single cell level. / Berman, E.; Rubin, B. Y.; Broxmeyer, Hal.

In: Cancer Research, Vol. 46, No. 7, 1986, p. 3309-3312.

Research output: Contribution to journalArticle

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abstract = "U937 cells, an established monoblast or early monocyte cell line, were assessed as a model in vitro for the regulation of cell growth at the single cell level. Colony formation by 500 U937 cells, preinduced to a state of responsiveness to lactoferrin (LF) by incubation with human γ interferon was suppressed by LF. LF-suppressed colony formation was restored by partially purified growth activity derived from U937 cells. The release of growth factor(s) into conditioned medium required concentrations of >500 U937 cells/ml and this release was dependent on the length of time that the cells conditioned the culture medium. This release was suppressed by LF. U937 cells were induced to a state of responsiveness to LF by incubation with human γ interferon, washed, and plated as a single cell per well. Individual cells formed colonies with a cloning efficiency of ~50{\%} which equalled the cloning efficiency detected when 500 U937 cells/ml were plated, suggesting that U937 colony forming cells might contain endogenous growth activity. Detection of these endogenous growth activities required the use of LF. The cloning efficiency of individually isolated U937 cells was suppressed by ~50{\%} with LF, similar to the LF suppression of colony formation when 500 cells/ml were plated. That the LF-suppressed U937 colony forming cells required growth activity was suggested as the cloning efficiency of LF-suppressed individually isolated U937 colony forming cells was restored by partially purified U937 growth activity. Partially purified U937 growth activity did not stimulate, enhance, or inhibit colony formation by normal human bone marrow granulocyte-macrophage progenitors. U937 cells can thus serve as a useful model for the study of growth regulation at the level of a single cell.",
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AU - Rubin, B. Y.

AU - Broxmeyer, Hal

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N2 - U937 cells, an established monoblast or early monocyte cell line, were assessed as a model in vitro for the regulation of cell growth at the single cell level. Colony formation by 500 U937 cells, preinduced to a state of responsiveness to lactoferrin (LF) by incubation with human γ interferon was suppressed by LF. LF-suppressed colony formation was restored by partially purified growth activity derived from U937 cells. The release of growth factor(s) into conditioned medium required concentrations of >500 U937 cells/ml and this release was dependent on the length of time that the cells conditioned the culture medium. This release was suppressed by LF. U937 cells were induced to a state of responsiveness to LF by incubation with human γ interferon, washed, and plated as a single cell per well. Individual cells formed colonies with a cloning efficiency of ~50% which equalled the cloning efficiency detected when 500 U937 cells/ml were plated, suggesting that U937 colony forming cells might contain endogenous growth activity. Detection of these endogenous growth activities required the use of LF. The cloning efficiency of individually isolated U937 cells was suppressed by ~50% with LF, similar to the LF suppression of colony formation when 500 cells/ml were plated. That the LF-suppressed U937 colony forming cells required growth activity was suggested as the cloning efficiency of LF-suppressed individually isolated U937 colony forming cells was restored by partially purified U937 growth activity. Partially purified U937 growth activity did not stimulate, enhance, or inhibit colony formation by normal human bone marrow granulocyte-macrophage progenitors. U937 cells can thus serve as a useful model for the study of growth regulation at the level of a single cell.

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