Regulation of transforming growth factor-α mRNA expression in T3M4human pancreatic carcinoma cells

Betty J. Glinsmann-Gibson, Murray Korc

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Cultured human pancreatic cancer cells produce transforming growth factor-α (TGF-α), a potent mitogenic polypeptide. In the present study, we investigated the regulation of TGF-α mRNA expression in T3M4human pancreatic carcinoma cells. TGF-α mRNA levels were quantitated by densi-tometric analysis of autoradiographs obtained following hybridization of size-fractionated cytoplasmic RNA with32P-labeled cRNA coding for human TGF-α. There was a twofold increase in TGF-α mRNA levels at 2 h following addition of either epidermal growth factor (EGF) or TGF-α. However, TGF-α mRNA levels declined to near basal levels by 10 h. At 2 h, one-half maximal stimulation of TGF-α mRNA levels occurred at 1 nM and maximal stimulation at 4 nM of either EGF or TGF-α. The transcriptional inhibitor actinomycin D (Act D) and the phorbol ester, 12-0-tetradecanoyl-phorbol-13-αcetate (TPA), mimicked the actions of EGF and TGF-α. These findings indicate that the regulation of TGF-α mRNA expression in T3M4cells is complex, and is me-diated, in part, via the EGF receptor.

Original languageEnglish (US)
Pages (from-to)142-149
Number of pages8
JournalPancreas
Volume6
Issue number2
StatePublished - 1991
Externally publishedYes

Fingerprint

Transforming Growth Factors
Messenger RNA
Epidermal Growth Factor
Pancreatic Carcinoma
Complementary RNA
Dactinomycin
Phorbol Esters
Pancreatic Neoplasms
Epidermal Growth Factor Receptor
RNA
Peptides

Keywords

  • Autocrine regulation
  • Epidermal growth factor receptor
  • Pancreatic cancer
  • Transforming growth factor-α

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Internal Medicine
  • Gastroenterology

Cite this

Regulation of transforming growth factor-α mRNA expression in T3M4human pancreatic carcinoma cells. / Glinsmann-Gibson, Betty J.; Korc, Murray.

In: Pancreas, Vol. 6, No. 2, 1991, p. 142-149.

Research output: Contribution to journalArticle

Glinsmann-Gibson, Betty J. ; Korc, Murray. / Regulation of transforming growth factor-α mRNA expression in T3M4human pancreatic carcinoma cells. In: Pancreas. 1991 ; Vol. 6, No. 2. pp. 142-149.
@article{f691cc0cc9a94e92b9ca59c2a366b177,
title = "Regulation of transforming growth factor-α mRNA expression in T3M4human pancreatic carcinoma cells",
abstract = "Cultured human pancreatic cancer cells produce transforming growth factor-α (TGF-α), a potent mitogenic polypeptide. In the present study, we investigated the regulation of TGF-α mRNA expression in T3M4human pancreatic carcinoma cells. TGF-α mRNA levels were quantitated by densi-tometric analysis of autoradiographs obtained following hybridization of size-fractionated cytoplasmic RNA with32P-labeled cRNA coding for human TGF-α. There was a twofold increase in TGF-α mRNA levels at 2 h following addition of either epidermal growth factor (EGF) or TGF-α. However, TGF-α mRNA levels declined to near basal levels by 10 h. At 2 h, one-half maximal stimulation of TGF-α mRNA levels occurred at 1 nM and maximal stimulation at 4 nM of either EGF or TGF-α. The transcriptional inhibitor actinomycin D (Act D) and the phorbol ester, 12-0-tetradecanoyl-phorbol-13-αcetate (TPA), mimicked the actions of EGF and TGF-α. These findings indicate that the regulation of TGF-α mRNA expression in T3M4cells is complex, and is me-diated, in part, via the EGF receptor.",
keywords = "Autocrine regulation, Epidermal growth factor receptor, Pancreatic cancer, Transforming growth factor-α",
author = "Glinsmann-Gibson, {Betty J.} and Murray Korc",
year = "1991",
language = "English (US)",
volume = "6",
pages = "142--149",
journal = "Pancreas",
issn = "0885-3177",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Regulation of transforming growth factor-α mRNA expression in T3M4human pancreatic carcinoma cells

AU - Glinsmann-Gibson, Betty J.

AU - Korc, Murray

PY - 1991

Y1 - 1991

N2 - Cultured human pancreatic cancer cells produce transforming growth factor-α (TGF-α), a potent mitogenic polypeptide. In the present study, we investigated the regulation of TGF-α mRNA expression in T3M4human pancreatic carcinoma cells. TGF-α mRNA levels were quantitated by densi-tometric analysis of autoradiographs obtained following hybridization of size-fractionated cytoplasmic RNA with32P-labeled cRNA coding for human TGF-α. There was a twofold increase in TGF-α mRNA levels at 2 h following addition of either epidermal growth factor (EGF) or TGF-α. However, TGF-α mRNA levels declined to near basal levels by 10 h. At 2 h, one-half maximal stimulation of TGF-α mRNA levels occurred at 1 nM and maximal stimulation at 4 nM of either EGF or TGF-α. The transcriptional inhibitor actinomycin D (Act D) and the phorbol ester, 12-0-tetradecanoyl-phorbol-13-αcetate (TPA), mimicked the actions of EGF and TGF-α. These findings indicate that the regulation of TGF-α mRNA expression in T3M4cells is complex, and is me-diated, in part, via the EGF receptor.

AB - Cultured human pancreatic cancer cells produce transforming growth factor-α (TGF-α), a potent mitogenic polypeptide. In the present study, we investigated the regulation of TGF-α mRNA expression in T3M4human pancreatic carcinoma cells. TGF-α mRNA levels were quantitated by densi-tometric analysis of autoradiographs obtained following hybridization of size-fractionated cytoplasmic RNA with32P-labeled cRNA coding for human TGF-α. There was a twofold increase in TGF-α mRNA levels at 2 h following addition of either epidermal growth factor (EGF) or TGF-α. However, TGF-α mRNA levels declined to near basal levels by 10 h. At 2 h, one-half maximal stimulation of TGF-α mRNA levels occurred at 1 nM and maximal stimulation at 4 nM of either EGF or TGF-α. The transcriptional inhibitor actinomycin D (Act D) and the phorbol ester, 12-0-tetradecanoyl-phorbol-13-αcetate (TPA), mimicked the actions of EGF and TGF-α. These findings indicate that the regulation of TGF-α mRNA expression in T3M4cells is complex, and is me-diated, in part, via the EGF receptor.

KW - Autocrine regulation

KW - Epidermal growth factor receptor

KW - Pancreatic cancer

KW - Transforming growth factor-α

UR - http://www.scopus.com/inward/record.url?scp=0025980638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025980638&partnerID=8YFLogxK

M3 - Article

C2 - 1886882

AN - SCOPUS:0025980638

VL - 6

SP - 142

EP - 149

JO - Pancreas

JF - Pancreas

SN - 0885-3177

IS - 2

ER -