Regulatory mechanisms in bone following mechanical loading

Sara M. Mantila Roosa, Charles H. Turner, Yunlong Liu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Bone responds with increased bone formation to mechanical loading, and the time course of bone formation after initiating mechanical loading is well characterized. However, the regulatory activities governing the loading-dependent changes in gene expression are not well understood. The goal of this study was to identify the time-dependent regulatory mechanisms that governed mechanical loading-induced gene expression in bone using a predictive bioinformatics algorithm. A standard model for bone loading in rodents was employed in which the right forelimb was loaded axially for three minutes per day, while the left forearm served as a non-loaded, contralateral control. Animals were subjected to loading sessions every day, with 24 hours between sessions. Ulnas were sampled at 11 time points, from 4 hours to 32 days after beginning loading. Using a predictive bioinformatics algorithm, we created a linear model of gene expression and identified 44 transcription factor binding motifs and 29 microRNA binding sites that were predicted to regulate gene expression across the time course. Known and novel transcription factor binding motifs were identified throughout the time course, as were several novel microRNA binding sites. These time-dependent regulatory mechanisms may be important in controlling the loading-induced bone formation process.

Original languageEnglish
Pages (from-to)43-53
Number of pages11
JournalGene Regulation and Systems Biology
Volume2012
Issue number6
StatePublished - 2012

Fingerprint

bone
Bone
bone formation
bones
Bone and Bones
gene expression
microRNA
Osteogenesis
bioinformatics
Gene expression
Gene Expression
binding sites
transcription factors
Computational Biology
MicroRNAs
ulna
Transcription factors
Transcription Factors
Binding sites
Bioinformatics

Keywords

  • Bone
  • Exon array
  • Mechanical loading
  • MicroRNA
  • Regulation
  • Transcription factor

ASJC Scopus subject areas

  • Genetics
  • Ecology, Evolution, Behavior and Systematics
  • Computer Science Applications
  • Molecular Biology

Cite this

Regulatory mechanisms in bone following mechanical loading. / Mantila Roosa, Sara M.; Turner, Charles H.; Liu, Yunlong.

In: Gene Regulation and Systems Biology, Vol. 2012, No. 6, 2012, p. 43-53.

Research output: Contribution to journalArticle

Mantila Roosa, SM, Turner, CH & Liu, Y 2012, 'Regulatory mechanisms in bone following mechanical loading', Gene Regulation and Systems Biology, vol. 2012, no. 6, pp. 43-53.
Mantila Roosa, Sara M. ; Turner, Charles H. ; Liu, Yunlong. / Regulatory mechanisms in bone following mechanical loading. In: Gene Regulation and Systems Biology. 2012 ; Vol. 2012, No. 6. pp. 43-53.
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