Relation between cellular repolarization characteristics and critical mass for human ventricular fibrillation

Tsu Juey Wu, Masaaki Yashima, Rahul Doshi, Young Hoon Kim, Charles A. Athill, James J C Ong, Lawrence Czer, Alfredo Trento, Carlos Blanche, Robert M. Kass, Alan Garfinkel, James N. Weiss, Michael C. Fishbein, Hrayr S. Karagueuzian, Peng-Sheng Chen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Introduction: The critical mass for human ventricular fibrillation (VF) and its electrical determinants are unclear. The goal of this study was to evaluate the relationship between repolarization characteristics and critical mass for VF in diseased human cardiac tissues. Methods and Results: Eight native hearts from transplant recipients were studied. The right ventricle was immediately excised, then perfused (n = 6) or superfused (n = 2) with Tyrode's solution at 36°C. The action potential duration (APD) restitution curve was determined by an S1-S2 method. Programmed stimulation and burst pacing were used to induce VF. In 3 of 8 tissues, 10 μM cromakalim, an ATP- sensitive potassium channel opener, was added to the perfusate and the stimulation protocol repeated. Results show that, at baseline, VF did not occur either spontaneously or during rewarming, and it could not be induced by aggressive electrical stimulation in any tissue. The mean APD at 90% depolarization (APD90) at a cycle length of 600 msec was 227 ± 49 msec, and the mean slope of the APD restitution curve was 0.22 ± 0.08. Among the six tissues perfused, five were not treated with any antiarrhythmic agent. The weight of these five heart samples averaged 111 ± 23 g (range 85 to 138). However, after cromakalim infusion, sustained VF (> 30 min in duration) was consistently induced. As compared with baseline in the same tissues, cromakalim shortened the APD90 from 243 ± 32 msec to 55 ± 18 msec (P <0.001) and increased the maximum slope of the APD restitution curve from 0.24 ± 0.11 to 1.43 ± 0.10 (P <0.01). Conclusion: At baseline, the critical mass for VF in diseased human hearts in vitro is >111 g. However, the critical mass for VF can vary, as it can be reduced by shortening APD and increasing the slope of the APD restitution curve.

Original languageEnglish (US)
Pages (from-to)1077-1089
Number of pages13
JournalJournal of Cardiovascular Electrophysiology
Volume10
Issue number8
StatePublished - 1999
Externally publishedYes

Fingerprint

Ventricular Fibrillation
Action Potentials
Cromakalim
pamidronate
Rewarming
KATP Channels
Electric Stimulation
Heart Ventricles
Heart Diseases
Weights and Measures

Keywords

  • Action potential duration
  • Action potential duration restitution curve
  • Critical mass
  • Cromakalim
  • Ventricular fibrillation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology

Cite this

Relation between cellular repolarization characteristics and critical mass for human ventricular fibrillation. / Wu, Tsu Juey; Yashima, Masaaki; Doshi, Rahul; Kim, Young Hoon; Athill, Charles A.; Ong, James J C; Czer, Lawrence; Trento, Alfredo; Blanche, Carlos; Kass, Robert M.; Garfinkel, Alan; Weiss, James N.; Fishbein, Michael C.; Karagueuzian, Hrayr S.; Chen, Peng-Sheng.

In: Journal of Cardiovascular Electrophysiology, Vol. 10, No. 8, 1999, p. 1077-1089.

Research output: Contribution to journalArticle

Wu, TJ, Yashima, M, Doshi, R, Kim, YH, Athill, CA, Ong, JJC, Czer, L, Trento, A, Blanche, C, Kass, RM, Garfinkel, A, Weiss, JN, Fishbein, MC, Karagueuzian, HS & Chen, P-S 1999, 'Relation between cellular repolarization characteristics and critical mass for human ventricular fibrillation', Journal of Cardiovascular Electrophysiology, vol. 10, no. 8, pp. 1077-1089.
Wu, Tsu Juey ; Yashima, Masaaki ; Doshi, Rahul ; Kim, Young Hoon ; Athill, Charles A. ; Ong, James J C ; Czer, Lawrence ; Trento, Alfredo ; Blanche, Carlos ; Kass, Robert M. ; Garfinkel, Alan ; Weiss, James N. ; Fishbein, Michael C. ; Karagueuzian, Hrayr S. ; Chen, Peng-Sheng. / Relation between cellular repolarization characteristics and critical mass for human ventricular fibrillation. In: Journal of Cardiovascular Electrophysiology. 1999 ; Vol. 10, No. 8. pp. 1077-1089.
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abstract = "Introduction: The critical mass for human ventricular fibrillation (VF) and its electrical determinants are unclear. The goal of this study was to evaluate the relationship between repolarization characteristics and critical mass for VF in diseased human cardiac tissues. Methods and Results: Eight native hearts from transplant recipients were studied. The right ventricle was immediately excised, then perfused (n = 6) or superfused (n = 2) with Tyrode's solution at 36°C. The action potential duration (APD) restitution curve was determined by an S1-S2 method. Programmed stimulation and burst pacing were used to induce VF. In 3 of 8 tissues, 10 μM cromakalim, an ATP- sensitive potassium channel opener, was added to the perfusate and the stimulation protocol repeated. Results show that, at baseline, VF did not occur either spontaneously or during rewarming, and it could not be induced by aggressive electrical stimulation in any tissue. The mean APD at 90{\%} depolarization (APD90) at a cycle length of 600 msec was 227 ± 49 msec, and the mean slope of the APD restitution curve was 0.22 ± 0.08. Among the six tissues perfused, five were not treated with any antiarrhythmic agent. The weight of these five heart samples averaged 111 ± 23 g (range 85 to 138). However, after cromakalim infusion, sustained VF (> 30 min in duration) was consistently induced. As compared with baseline in the same tissues, cromakalim shortened the APD90 from 243 ± 32 msec to 55 ± 18 msec (P <0.001) and increased the maximum slope of the APD restitution curve from 0.24 ± 0.11 to 1.43 ± 0.10 (P <0.01). Conclusion: At baseline, the critical mass for VF in diseased human hearts in vitro is >111 g. However, the critical mass for VF can vary, as it can be reduced by shortening APD and increasing the slope of the APD restitution curve.",
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T1 - Relation between cellular repolarization characteristics and critical mass for human ventricular fibrillation

AU - Wu, Tsu Juey

AU - Yashima, Masaaki

AU - Doshi, Rahul

AU - Kim, Young Hoon

AU - Athill, Charles A.

AU - Ong, James J C

AU - Czer, Lawrence

AU - Trento, Alfredo

AU - Blanche, Carlos

AU - Kass, Robert M.

AU - Garfinkel, Alan

AU - Weiss, James N.

AU - Fishbein, Michael C.

AU - Karagueuzian, Hrayr S.

AU - Chen, Peng-Sheng

PY - 1999

Y1 - 1999

N2 - Introduction: The critical mass for human ventricular fibrillation (VF) and its electrical determinants are unclear. The goal of this study was to evaluate the relationship between repolarization characteristics and critical mass for VF in diseased human cardiac tissues. Methods and Results: Eight native hearts from transplant recipients were studied. The right ventricle was immediately excised, then perfused (n = 6) or superfused (n = 2) with Tyrode's solution at 36°C. The action potential duration (APD) restitution curve was determined by an S1-S2 method. Programmed stimulation and burst pacing were used to induce VF. In 3 of 8 tissues, 10 μM cromakalim, an ATP- sensitive potassium channel opener, was added to the perfusate and the stimulation protocol repeated. Results show that, at baseline, VF did not occur either spontaneously or during rewarming, and it could not be induced by aggressive electrical stimulation in any tissue. The mean APD at 90% depolarization (APD90) at a cycle length of 600 msec was 227 ± 49 msec, and the mean slope of the APD restitution curve was 0.22 ± 0.08. Among the six tissues perfused, five were not treated with any antiarrhythmic agent. The weight of these five heart samples averaged 111 ± 23 g (range 85 to 138). However, after cromakalim infusion, sustained VF (> 30 min in duration) was consistently induced. As compared with baseline in the same tissues, cromakalim shortened the APD90 from 243 ± 32 msec to 55 ± 18 msec (P <0.001) and increased the maximum slope of the APD restitution curve from 0.24 ± 0.11 to 1.43 ± 0.10 (P <0.01). Conclusion: At baseline, the critical mass for VF in diseased human hearts in vitro is >111 g. However, the critical mass for VF can vary, as it can be reduced by shortening APD and increasing the slope of the APD restitution curve.

AB - Introduction: The critical mass for human ventricular fibrillation (VF) and its electrical determinants are unclear. The goal of this study was to evaluate the relationship between repolarization characteristics and critical mass for VF in diseased human cardiac tissues. Methods and Results: Eight native hearts from transplant recipients were studied. The right ventricle was immediately excised, then perfused (n = 6) or superfused (n = 2) with Tyrode's solution at 36°C. The action potential duration (APD) restitution curve was determined by an S1-S2 method. Programmed stimulation and burst pacing were used to induce VF. In 3 of 8 tissues, 10 μM cromakalim, an ATP- sensitive potassium channel opener, was added to the perfusate and the stimulation protocol repeated. Results show that, at baseline, VF did not occur either spontaneously or during rewarming, and it could not be induced by aggressive electrical stimulation in any tissue. The mean APD at 90% depolarization (APD90) at a cycle length of 600 msec was 227 ± 49 msec, and the mean slope of the APD restitution curve was 0.22 ± 0.08. Among the six tissues perfused, five were not treated with any antiarrhythmic agent. The weight of these five heart samples averaged 111 ± 23 g (range 85 to 138). However, after cromakalim infusion, sustained VF (> 30 min in duration) was consistently induced. As compared with baseline in the same tissues, cromakalim shortened the APD90 from 243 ± 32 msec to 55 ± 18 msec (P <0.001) and increased the maximum slope of the APD restitution curve from 0.24 ± 0.11 to 1.43 ± 0.10 (P <0.01). Conclusion: At baseline, the critical mass for VF in diseased human hearts in vitro is >111 g. However, the critical mass for VF can vary, as it can be reduced by shortening APD and increasing the slope of the APD restitution curve.

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KW - Action potential duration restitution curve

KW - Critical mass

KW - Cromakalim

KW - Ventricular fibrillation

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