Relation of the Escherichia coli DNAx gene to its two products-the τ and γ subunits of DNA polymerase III holoenzyme

Suk-Hee Lee, Patrick Kanda, Ronald C. Kennedy, James R. Walker

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The Escherichia coli DNA polymerase III holoenzyme 71.1 kDa τ subunit is a 643 amino acid protein encoded by the dnaX gene. This gene also encodes the holoenzyme 56.5 kDa γ subunit. The τ factor (as a τ' -LacZ' fusion protein) has been isolated and shown to be cleaved in vitro to form γ and a 135 kda C-terminal cleavage product. The τ' -LacZ' fusion protein, γ, and the C-terminal cleavage product have been isolated. N-terminal sequencing has demonstrated that τ and γ share the same N-terminal sequences and that τ is proteolytically cleaved in vitro between residues 498 and 499 to form γ. In addition, residues 420-440 were shown to be present in both τ and γ by use of antibody specific for a synthetic peptide corresponding to that sequence. Some mechanism functions in vivo to ensure that τ and γ are synthesized in a ratio of about one-to-one, as shown by radioimmune precipitation of τ and γ from cellular extracts.

Original languageEnglish (US)
Pages (from-to)7663-7675
Number of pages13
JournalNucleic Acids Research
Volume15
Issue number19
DOIs
StatePublished - Oct 12 1987
Externally publishedYes

Fingerprint

DNA Polymerase III
Holoenzymes
Escherichia coli
Escherichia Coli
DNA
Fusion reactions
Genes
Gene
Proteins
Protein
Fusion
Protein C
Antibody
Amino Acids
Antibodies
Peptides
Sequencing
Amino acids
In Vitro Techniques
Form

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Relation of the Escherichia coli DNAx gene to its two products-the τ and γ subunits of DNA polymerase III holoenzyme. / Lee, Suk-Hee; Kanda, Patrick; Kennedy, Ronald C.; Walker, James R.

In: Nucleic Acids Research, Vol. 15, No. 19, 12.10.1987, p. 7663-7675.

Research output: Contribution to journalArticle

Lee, Suk-Hee ; Kanda, Patrick ; Kennedy, Ronald C. ; Walker, James R. / Relation of the Escherichia coli DNAx gene to its two products-the τ and γ subunits of DNA polymerase III holoenzyme. In: Nucleic Acids Research. 1987 ; Vol. 15, No. 19. pp. 7663-7675.
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